Human ENPP3 / CD203c Protein, His Tag (active enzyme)

分子別名（Synonym）

ENPP3,CD203c,NPP3,E-NPP3,PD-Ibeta,NPPase,PDNP3

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human ENPP3, His Tag (EN3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 48 - Ile 875 (Accession # O14638-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 96.9 kDa. The protein migrates as 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

NPP家族包含七名成員，根據(jù)其底物可分為兩組。第一類包括核苷酸降解蛋白NPP1、3和4。NPP3（CD203c，ENPP3）在多個器官中表達(dá)，包括上皮和粘膜表面，尤其是嗜堿性粒細(xì)胞和肥大細(xì)胞?？乖Y(jié)合的IgE激活嗜堿性粒細(xì)胞導(dǎo)致炎癥介質(zhì)的釋放和NPP3向細(xì)胞表面的快速上調(diào)。事實上，這種蛋白質(zhì)是通過流式細(xì)胞術(shù)診斷患者嗜堿性粒細(xì)胞過敏原敏感性的常見標(biāo)志物。嗜堿性粒細(xì)胞和肥大細(xì)胞介導(dǎo)對某些病原體的反應(yīng)，以及急性和慢性過敏反應(yīng)。激活后，這些細(xì)胞釋放ATP，ATP以自分泌的方式進(jìn)一步刺激它們。NPP3上調(diào)有助于降解ATP并抑制慢性過敏性炎癥（但不是急性反應(yīng)）。