Human MUC17 Protein, His Tag

分子別名（Synonym）

Mucin-17,MUC-17,MUC17,MUC-3,MUC3

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human MUC-17, His Tag (MU7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 4131 - Leu 4390 (Accession # Q685J3-1).

Predicted N-terminus: Arg 4131 & 4244 Ser

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein was cleaved within the SEA domain between 4243 Gly and 4244 Ser, and was cleaved into N and C-terminal fragment with calculated MW of 12.6 kDa and 18.6 kDa respectively. The protein migrates as 20 kDa and 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

膜粘蛋白在上皮細(xì)胞中具有多種功能，包括細(xì)胞保護(hù)、轉(zhuǎn)移過程中的外滲、維持管腔結(jié)構(gòu)和信號(hào)轉(zhuǎn)導(dǎo)。MUC17包含一個(gè)延伸的、重復(fù)的細(xì)胞外糖基化結(jié)構(gòu)域和一個(gè)羧基末端，羧基末端有兩個(gè)EGF樣結(jié)構(gòu)域、一個(gè)SEA模塊結(jié)構(gòu)域、另一個(gè)跨膜結(jié)構(gòu)域和具有潛在絲氨酸和酪氨酸磷酸化位點(diǎn)的細(xì)胞質(zhì)結(jié)構(gòu)域。通過其C末端與PDZK1相互作用，這種相互作用對(duì)于正確定位非常重要。可能在維持粘膜表面的穩(wěn)態(tài)方面發(fā)揮作用。