Human SOST / Sclerostin Protein, His Tag

分子別名（Synonym）

SOST,VBCH

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human SOST, His Tag (HST-H5245) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Sclerostin（SOST）也被稱為Sclerosteosis，VBCH，是一種分泌性糖蛋白，具有用于分泌的信號(hào)肽和C末端半胱氨酸結(jié)樣（CTCK）結(jié)構(gòu)域，屬于Cerberus/DAN家族的骨形態(tài)發(fā)生蛋白（BMP）拮抗劑。硬骨素由骨細(xì)胞產(chǎn)生，對(duì)骨形成具有抗合成代謝作用。最近，硬骨素已被鑒定為與LRP5/6受體結(jié)合并抑制Wnt信號(hào)通路。在這些情況下，Wnt通路的抑制對(duì)骨形成具有拮抗作用（即硬骨素拮抗骨形成）。已經(jīng)表明，SOST以高親和力結(jié)合BMP-5、-6和-7，以低親和力結(jié)合BMP-2和-4。甲狀旁腺激素、機(jī)械負(fù)荷和包括抑瘤素M、心肌營(yíng)養(yǎng)素-1和白血病抑制因子在內(nèi)的細(xì)胞因子抑制了骨細(xì)胞產(chǎn)生硬化素。降鈣素可以增加硬化素的產(chǎn)生。因此，成骨細(xì)胞的活性是由負(fù)反饋系統(tǒng)自我調(diào)節(jié)的。硬骨素突變與硬骨癥綜合征有關(guān)，硬骨素表達(dá)減少導(dǎo)致一種較輕的疾病，稱為van Buchem病。