中文名稱:CTAB植物基因組DNA快速提取試劑盒 | 英文名稱:Modified CTAB Plant DNA Kit |
品牌: 艾德萊 | 產(chǎn)地: 北京 |
保存條件: 常溫 | 產(chǎn)品類別: DNA提取 |
貨號: DN1402 | 規(guī)格: 100次 |
是否進口: 否 |
DN14-CTAB植物基因組DNA快速提取試劑盒
產(chǎn)品貨號 | 產(chǎn)品名稱 | 規(guī)格 | 報價 | 特價 |
DN1402 | CTAB植物基因組DNA快速提取試劑盒 | 100次 | 640 | 448 |
產(chǎn)品介紹:
改進的經(jīng)典CTAB植物DNA抽提液內(nèi)(添加多種針對植物特點的多糖、多酚去除成份)迅速裂解細胞和滅活細胞內(nèi)核酸酶,氯仿抽提后通過離心**多糖、多酚和蛋白質(zhì)(根據(jù)需要,上清中還加入異丙醇離心沉淀基因組DNA,進一步去除其它各種雜質(zhì)),然后基因組DNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜,再通過一系列快速的漂洗-離心的步驟, 進一步將多糖、多酚和細胞代謝物、蛋白等雜質(zhì)去除,**低鹽的洗脫緩沖液將純凈基因組DNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進口特制吸附膜,柱與柱之間吸附量差異極小,可重復性好??朔藝a(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。
2.不需要使用有毒的苯酚等試劑,也不需要乙醇沉淀等步驟。
3.快速,簡捷,單個樣品操作一般可在1小時內(nèi)完成。
4.數(shù)種去多糖、多酚成份和多次柱漂洗確保高純度,OD260/OD280典型的比值達1.7~1.9,長度可達30 kb -50kb,可直接用于PCR,Southern-blot和各種酶切反應。
使用該產(chǎn)品發(fā)表的部分文章:
1.Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, DOI 10.1007/s00299-013-1413-6
2.Taxonomy and phylogeny of Ceriporia (Polyporales, Basidiomycota) with an emphasis of Chinese collections. Mycol Progress 2014, 13: 81–93
3.Phlebiporia bubalina gen. et. sp. nov. (Meruliaceae, Polyporales) from southwest China with a preliminary
4.Down-Regulated miR-30a in Clear Cell Renal Cell Carcinoma Correlated with Tumor Hematogenous Metastasis by Targeting Angiogenesis-Specific DLL4. miR-30a Targets DLL4 in RCC Metastasis. June 2013 | Volume 8 | Issue 6 | e67294
5.A preliminary report on decay and canker of Acacia richii caused by Inonotus rickii in China. For. Path. 44 (2014) 82–84 . doi: 10.1111/efp.12088. 2014 Blackwell Verlag GmbH.
6.Hispidaedalea gen. nov. and Griseoporia taiwanense sp. nov. (Gloeophyllales, Basidiomycota) based on morphological and molecular characters. ORIGINAL ARTICLE. Mycol Progress. DOI 10.1007/s11557-014-0966-2.
7.Two new species of the Heterobasidion insulare complex based on morphology and molecular data. Mycoscience 2014, 55(4): 289–298
8.Three homologous genes encoding functional D8-sphingolipid desaturase in Populus tomentosa. Genes Genom 2014, 36: 293–301
9.Isolation, Characterization, and Structure Analysis of a Vacuolar Processing Enzyme Gene (MhVPEγ) from Malus hupehensis (Pamp) Rehd. Appl Biochem Biotechnol 2014, 73: 579–5954
10.Morphological characters and molecular data reveal a new species of Fomitopsis (Polyporales) from southern China. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.05.0044
11.Morphology and molecular phylogeny for two new species of Fomitopsis (Basidiomycota) from South China. Mycol Progress 2014, 13: 905–914
12.Coltriciella globosa and C. pseudodependens spp. nov. (Hymenochaetales) from southern China based on morphological and molecular characters. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.06.001
13.Contribution of arbuscular mycorrhizal fungi of sedges to soil aggregation along an altitudinal alpine grassland gradient on the Tibetan Plateau. Environmental Microbiology 2015, (17)8:2841–2857
14.Stability Assay of Three DNA Extraction Methods for Detection of Strawberry Vein Banding Virus. Acta Agriculturae Boreali-Sinica 2015, 30(3):200-204
成立日期 | 2009-01-13 (17年) | 注冊資本 | 500萬元 |
員工人數(shù) | 10-50人 | 年營業(yè)額 | ¥ 100萬-300萬 |
主營行業(yè) | 化學試劑 | 經(jīng)營模式 | 貿(mào)易,試劑,服務 |
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