Biotinylated Human PCSK9 Protein, Avitag?,His Tag (MALS verified)

分子別名（Synonym）

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).

Predicted N-terminus: Gln 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through noncovalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 63-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology.

Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

前蛋白轉(zhuǎn)化酶枯草桿菌蛋白酶/kexin 9型（PCSK9）也稱(chēng)為NARC1（神經(jīng)細(xì)胞凋亡調(diào)節(jié)轉(zhuǎn)化酶），是一種新發(fā)現(xiàn)的屬于肽酶S8亞家族的枯草酶。小鼠PCSK9被合成為可溶性酶原，并在內(nèi)質(zhì)網(wǎng)中進(jìn)行自催化分子內(nèi)處理，導(dǎo)致其前肽的切割，該前肽與分泌的活性酶保持關(guān)聯(lián)，具有寬的堿性最佳pH值。這種蛋白質(zhì)在膽固醇穩(wěn)態(tài)中起著重要的調(diào)節(jié)作用。PCSK9與低密度脂蛋白受體（LDLR）的表皮生長(zhǎng)因子樣重復(fù)序列A（EGF-A）結(jié)構(gòu)域結(jié)合，誘導(dǎo)LDLR降解。PCSK9也可能在皮層神經(jīng)元的分化中發(fā)揮作用。該基因的突變與一種罕見(jiàn)的常染色體顯性家族性高膽固醇血癥（HCHOLA3）有關(guān)。