Human IL-13 Protein, His Tag, premium grade

分子別名（Synonym）

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-13 Protein, His Tag, premium grade (IL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).

Predicted N-terminus: Gly 21

Human IL-13 Protein, His Tag, premium grade (IL3-H52H4), designed for preclinical stage, has the same activity and performance with GMP Human IL-13 Protein, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa and 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素13（IL13）也稱為ALRH、BHR1和P600，是一種單鏈糖基化多肽，是調(diào)節(jié)炎癥和免疫反應(yīng)的關(guān)鍵細(xì)胞因子。IL13由許多細(xì)胞類型分泌，但特別是由T輔助型2（Th2）細(xì)胞分泌。IL-13通過多亞基受體誘導(dǎo)其作用，該受體包括IL-4受體的α鏈（IL-4Rα）和兩個(gè)已知的IL-13特異性結(jié)合鏈中的至少一個(gè)。IL-13的功能與IL-4的功能相當(dāng)重疊，特別是在造血細(xì)胞誘導(dǎo)的變化方面，但考慮到IL-4更有效的作用，這些作用可能不那么重要。IL-13誘導(dǎo)基質(zhì)金屬蛋白酶（MMPs），作為防止易窒息的過度過敏性炎癥機(jī)制的一部分。IL-13誘導(dǎo)過敏性肺病的許多特征，包括氣道高反應(yīng)性、杯狀細(xì)胞化生和粘液分泌過多，這些都會(huì)導(dǎo)致氣道阻塞。