Human FOLR1 Protein, His Tag (MALS verified)

分子別名（Synonym）

FOLR-1,FBP,FOLR,FRα

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human FOLR1, His Tag (FO1-H52H1) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).

Predicted N-terminus: Arg 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-40 KDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

葉酸受體1（FOLR1）也稱為葉酸受體α、葉酸結(jié)合蛋白（FBP）、FOLR，是葉酸受體（FOLR）家族的成員。該基因家族的成員對(duì)葉酸和幾種還原的葉酸衍生物具有高親和力，并介導(dǎo)5-甲基四氫葉酸向細(xì)胞內(nèi)部的遞送。成熟FOLR1是一種N-糖基化蛋白，通過GPI連接錨定在細(xì)胞表面。FOLR1主要在上皮細(xì)胞上表達(dá)，在許多癌癥上顯著上調(diào)。FOLR1被內(nèi)化到內(nèi)涵體系統(tǒng)中，在回收到細(xì)胞表面之前，它與配體解離?？扇苄孕问降?/span>FOLR1可以從細(xì)胞表面蛋白水解脫落到血清和母乳中。FOLR1的缺陷是由于腦葉酸轉(zhuǎn)運(yùn)缺乏（NCFTD）引起的神經(jīng)退行性變的原因。NCFTD是一種常染色體隱性遺傳疾病，由早期大腦特異性葉酸缺乏引起。