Human VEGF165-coupled Magnetic Beads
背景（Background）
人VEGF165偶聯(lián)磁珠是通過(guò)將生物素化的人VEGF165%蛋白偶聯(lián)到鏈親和素偶聯(lián)磁珠上來(lái)制備的。預(yù)偶聯(lián)珠可用于以高特異性從樣品中捕獲抗VEGF165抗體或VEGFR2/KDR蛋白。珠粒的均勻尺寸和大的表面積與體積比確保了在簡(jiǎn)單、快速、方便的工作流程中高效的免疫捕獲，并具有高的數(shù)據(jù)可重復(fù)性。
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).
應(yīng)用說(shuō)明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This is a non-sterile product.
The Isotype control (Cat. No. SMB-B01) is sold separately.
重構(gòu)方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存儲(chǔ)（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制劑（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
關(guān)鍵字： VEGF165磁珠;VEGF165 Beads;Magnetic Beads;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國(guó)生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國(guó)、美國(guó)、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長(zhǎng)期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。