IL-23 : IL-23R[Biotinylated] Inhibitor Screening ELISA Kit
背景（Background）
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
原理（Assay Principles）
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new Human IL-23 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated Human IL-23R to immobilized Human IL-23 in a functional ELISA assay and employs a simple colorimetric ELISA platform. Briefly, we provide you with a Human IL-23A&IL-12B protein, a Biotinylated Human IL-23R, an anti- IL-23A neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.
Your experiment will include 4 simple steps:
1) Coat the plate with Human IL-23A&IL-12B.
2) Add your molecule of interest to the tests.
3) Add Human IL-23R-Biotin to bind the coated Human IL-23A&IL-12B.
4) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
5) Finally, the half maximal inhibitory concentration (IC50) of your compound to Human IL-23A: Human IL-23R
binding will be determined by comparing OD readings among different experimental groups.
應用說明（Application）
This kit is developed for screening for inhibitors of human IL-23 binding to human IL-23R.
It is for research use only.
重構方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存儲（Storage）
Unopened kit should be stored at 2°C-8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
關鍵字： IL-23 ELISA Kit;IL-23試劑盒;IL-23 ELISA試劑盒;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。