中文名：5-溴-4-氯-3-吲哚基磷酸酯對(duì)甲苯胺鹽（BCIP)

英文名：5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

中文別名：X-磷酸鹽 對(duì)甲苯胺鹽

英文別名：6578-06-9|BCIP|5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt|BCIP p-toluidine salt|X-phosphate p-toluidine salt|p-toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate|5-Bromo-4-chloro-1H-indol-3-yl phosphate p-toluidine|5-Bromo-4-chloro-3-indolyl ph

純度：用于分子生物學(xué), ≥99%

貨號(hào)：B113076

包裝：100mg、500mg

Cas號(hào)：6578-06-9

存儲(chǔ)溫度：-20°C儲(chǔ)存,充氬

產(chǎn)品介紹：

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.堿性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑藍(lán)(NBT)用于比色法鑒定堿性磷酸酶標(biāo)記分子. BCIP/NBT 底物系統(tǒng)用途非常廣泛：包括 Northern Southern, Western blotting, 原位雜交（in situ hybridization）和免疫組織化學(xué)。 BCIP 可以有兩種鹽的形式: 二鈉鹽型溶于水，對(duì)甲苯胺型溶于二甲基甲酰胺（DMF）。這些鹽型可用于制備和NBT結(jié)合的儲(chǔ)存溶液和反應(yīng)緩沖液，構(gòu)成堿性磷酸酶的底物溶液。 底物系統(tǒng)和堿性磷酸酶一起培養(yǎng)，產(chǎn)生一種不溶的帶紫色的NBT雙甲臘( diformazan )產(chǎn)物，容易被觀察。

查看阿拉丁官網(wǎng)此產(chǎn)品相關(guān)對(duì)應(yīng)頁(yè)面：https://www.aladdin-e.com/zh_cn/B113076.html