LIF產(chǎn)品信息

英文名稱：Leukemia inhibitory factor

中文名稱：白血病抑制因子

靶點(diǎn)別稱：LIF,CDF,DIA,HILDA,MLPLI

物種：Human/Mouse/Rat

屬性：Protein

標(biāo)記：Biotin-labeled/Unconjugated

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

無菌（Sterility）

The sterility testing was performed by membrane filtration method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

LIF用戶評(píng)價(jià)

LIF分子背景

白血病抑制因子（LIF）是一種白細(xì)胞介素6類細(xì)胞因子，是細(xì)胞中影響細(xì)胞生長(zhǎng)和發(fā)育的蛋白質(zhì)。白血病抑制因子具有多種功能，如膽堿能神經(jīng)元分化、控制干細(xì)胞多能性、骨和脂肪代謝、因子依賴性細(xì)胞系的有絲分裂和促進(jìn)體內(nèi)巨核細(xì)胞的產(chǎn)生。

LIF的去除推動(dòng)干細(xì)胞分化，但它們保留了增殖潛力或多能性。因此，LIF用于小鼠胚胎干細(xì)胞培養(yǎng)。有必要將干細(xì)胞保持在未分化狀態(tài)，但是胚胎干細(xì)胞的基因操縱允許LIF非依賴性生長(zhǎng)，特別是Nanog基因的過表達(dá)。人胚胎干細(xì)胞培養(yǎng)不需要LIF。