3C (HRV)產(chǎn)品信息

英文名稱(chēng)：Picornain 3C protease

中文名稱(chēng)：Picornain 3C蛋白酶

靶點(diǎn)別稱(chēng)：HRV-3C Protease,3C Protease

物種：Human Rhinovirus

屬性：Protein

標(biāo)記：Unconjugated

產(chǎn)品描述（Product Details）

HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.

應(yīng)用說(shuō)明（Application）

Removal of purification tags from fusion proteins

單位定義（Unit Definition）

One unit will cleave >95% of 100 μg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.

質(zhì)量控制（Quility Control）

Endotoxin : Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH8.0 with trehalose as protectant.

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存儲(chǔ)（Storage）

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

3C (HRV)分子背景

Protease Recombinant is fusion protein of GST and human rhinovirus (HRV) type 14 3C protease.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.