PH20產(chǎn)品信息
英文名稱:T-cell surface antigen CD2
靶點(diǎn)別稱:SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15
物種:Human
標(biāo)簽:Tag Free / His Tag
屬性:Protein
標(biāo)記:Unconjugated
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.
PH20分子背景
透明質(zhì)酸酶PH20也稱為精子粘附分子1(SPAM1)和精子表面蛋白PH-20,屬于糖基水解酶56家族,SPAM1/PH-20在睪丸中表達(dá)。透明質(zhì)酸中N-乙?;?/span>-β-D-葡糖胺和D-葡萄糖醛酸殘基之間(1->4)鍵的SPAM-1/PH20隨機(jī)水解。SPAM-1/PH20參與精卵粘附。受精后,精子必須首先穿透卵子周圍的一層卵丘細(xì)胞,然后才能到達(dá)透明帶。卵丘細(xì)胞包埋在排卵前形成的含有透明質(zhì)酸的基質(zhì)中。SPAM1通過消化透明質(zhì)酸幫助穿透卵丘細(xì)胞層。
關(guān)鍵字: PH20;PH20蛋白;PH20重組蛋白;SPAM1;SPAM1蛋白;
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