RSV產(chǎn)品信息

物種：Human respiratory syncytial virus A (strain A2) / RSV-F0 / Human respiratory syncytial virus B (strain B1)

標(biāo)簽：His Tag & Avi Tag / His Tag

屬性：Protein / Kits

標(biāo)記：Biotin-labeled / FITC Labeled / Unconjugated

RSV蛋白

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Supplied as 0.2 μm filtered solution in PBS with trehalose as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲(chǔ)（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

RSV ELISA試劑盒

背景（Background）

Respiratory syncytial virus (RSV) is a highly contagious virus causing severe infection in infants and the elderly. Various approaches are being used to develop an effective RSV vaccine. The RSV fusion (F) subunit, particularly the cleaved trimeric pre-fusion F, is one of the most promising vaccine candidates under development.

A rapid and effective assay kit detecting the levels of HRSV (A) Pre-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.

應(yīng)用說(shuō)明（Application）

This kit is developed for Specific quantitative detection of HRSV (A) Pre-Fusion glycoprotein F0 in samples.

It is for research use only.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存儲(chǔ)（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Pre-Fusion glycoprotein F0 (RSV). The kit consists of Pre-coated Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody Microplate and Pre-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the Pre-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

RSV分子背景

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Both states exhibit epitopes targeted by neutralizing antibodies, and post-fusion RSV F is being developed as a vaccine candidate.

RSV用戶評(píng)價(jià)