中文名:磷酸酶(堿性)來源于小牛腸(純化)
英文名:Phosphatase, Alkaline from calf intestine(Purified)
中文別名:堿性磷酸酯酶
英文別名:Alkaline phosphatase|9001-78-9|Phosphatase, alkaline|ENTEROSTATIN,HUMAN
純度:≥3,000 units/mg protein(37℃,pH9.8,DEA)
貨號:P128628
包裝:1mg、5mg
Cas號:9001-78-9
存儲溫度:2-8°C儲存
產(chǎn)品介紹:
The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sμgars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.堿性磷酸酶可用于酪蛋白和其他蛋白質(zhì)的去磷酸化。堿性磷酸酶還可用于 DNA 或 RNA 的 5′-末端的去磷酸化,以防止自我連接。經(jīng)堿性磷酸酶的去磷酸化作用后,DNA 或 RNA 還可被放射性標(biāo)記磷酸鹽標(biāo)記(通過 T4 多核苷酸激酶)。
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關(guān)鍵字: 堿性磷酸酯酶;Alkaline phosphatase;9001-78-9;Phosphatase, alkaline
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