中文名：瘦素(人)(重組)

英文名：Leptin (human), (recombinant)

英文別名：OB gene product; OB Protein; Obesity Protein; OBS; Obesity factor

純度：≥97% (SDS-PAGE)

貨號：L356717

包裝：1mg、5mg

Cas號：177404-21-6

存儲溫度：-20°C儲存,干燥

產(chǎn)品介紹：

Leptin (aka OB protein, or Obesity factor protein) is a 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.|Leptin (human), (recombinant) is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16 kDa. The Leptin protein is purified by proprietary chromatographic techniques.|Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Product description

Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues.

Source

E.coli

Synonyms

Obesity protein (OB)

AA Sequence

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC

Purity:

≥ 97% by SDS-PAGE gel

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg

Accession Number

P41159

Gene ID

3952

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

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