應用
3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.
生化/生理作用
DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.
注意
Injecting a compatible solvent permits preparation of any desired strength solution without exposure.
重懸
Dissolving the contents in 100 mL of solvent yields a 0.1% solution.
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