產(chǎn)品詳情
產(chǎn)品描述:
This Human LOX ELISA Kit was designed for the quantitative measurement of Human LOX protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
別名:
lox ELISA Kit; LYOX ELISA Kit; LYOX_HUMAN ELISA Kit; Lysyl oxidase ELISA Kit; MGC105112 ELISA Kit; Protein lysine 6 oxidase ELISA Kit; Protein-lysine 6-oxidase ELISA Kit
縮寫:
LOX
種屬:
Homo sapiens (Human)
樣本類型:
serum, plasma, tissue homogenates
檢測范圍:
0.625 ng/mL-40 ng/mL
靈敏度:
0.156 ng/mL
反應(yīng)時間:
1-5h
樣本體積:
50-100ul
檢測波長:
450 nm
研究領(lǐng)域:
Signal Transduction
測定原理:
quantitative
測定方法:
Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
Three samples of known concentration were tested twenty times on one plate to assess. |
|
Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
|
|
|
|
|
|
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
|
1:1 | Average % | 97 |
|
Range % | 92-101 |
|
1:2 | Average % | 84 |
|
Range % | 80-88 |
|
1:4 | Average % | 90 |
|
Range % | 86-95 |
|
1:8 | Average % | 93 |
|
Range % | 86-98 |
|
回收率:
The recovery of human LOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range |
|
Serum (n=5) | 100 | 95-107 |
|
EDTA plasma (n=4) | 95 | 90-99 |
|
注意事項:
1、試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存
2、濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果
3、各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣
4、請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定計算時請最后乘以總稀釋倍數(shù)(xnx5)
5、封板膜只限一次性使用,以避免交叉污染
6、底物請避光保存。
7、嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn)
8、所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理
9、.本試劑不同批號組分不得混用。
艾普蒂生物 品牌保障:
1、質(zhì)量保證: 產(chǎn)品質(zhì)量穩(wěn)定,每批次產(chǎn)品必須通過QA、QC檢測才可出庫;
2、技術(shù)支持: 技術(shù)部5x8小時技術(shù)問題及時解答
3、服務(wù)周到: 發(fā)貨及時,三個工作日順豐快遞
4、售后無憂:產(chǎn)品質(zhì)量問題包退包換
艾普蒂ELISA試劑盒的優(yōu)勢:
1、高效、靈敏、特異的抗體;
2、穩(wěn)定的重復(fù)性和可靠性;
3、吸附性能好,空白值低,孔底透明度高的固相載體;
4、適用血清、血漿、組織勻漿液、細胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型!
關(guān)鍵字: Human LOX ELISA Kit;人賴氨酰氧化酶(LOX);人賴氨酰氧化酶 ELISA Kit;人 LOX ELISA KIT;
湖北艾普蒂生物工程有限公司(簡稱:艾普蒂)由在國內(nèi)科研試劑領(lǐng)域有著十幾年從業(yè)經(jīng)驗的專業(yè)技術(shù)團隊和企業(yè)管理團隊組建而成,專門從事以抗體、蛋白、細胞、化學(xué)品為核心的試劑產(chǎn)品研發(fā)與銷售。
我們長期聚焦于生命科學(xué)、生物醫(yī)藥、醫(yī)療診斷、分析檢測等領(lǐng)域,專注于為生命科學(xué)和生物技術(shù)領(lǐng)域的客戶提供優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)。提供生命科學(xué) 試劑、供應(yīng)鏈服務(wù)等,為更多的生命科學(xué)科研工作者提供 專業(yè)、全面、安全、快捷的產(chǎn)品和服務(wù)。產(chǎn)品覆蓋 基因、蛋白、細胞、組織、動物等水平的各種研究。
致力于成長為我國重要的生命科學(xué)試劑和成果轉(zhuǎn)化一體化的領(lǐng)導(dǎo)者、致力于成為中國大型的科研試劑產(chǎn)品供應(yīng)商之一。