產(chǎn)品詳情
產(chǎn)品描述:
This Human BMP4 ELISA Kit was designed for the quantitative measurement of Human BMP4 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
別名:
zgc:100779 ELISA Kit; BMP 2B ELISA Kit; BMP 4 ELISA Kit; BMP-2B ELISA Kit; BMP-4 ELISA Kit; BMP2B ELISA Kit; BMP2B1 ELISA Kit; BMP4 ELISA Kit; BMP4_HUMAN ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; Bone morphogenetic protein 4 ELISA Kit; DVR4 ELISA Kit; MCOPS6 ELISA Kit; MGC100779 ELISA Kit; OFC11 ELISA Kit; zbmp-4 ELISA Kit; ZYME ELISA Kit
縮寫:BMP4
種屬:
Homo sapiens (Human)
樣本類型:
serum, plasma, cell culture supernates
檢測(cè)范圍:
15.6 pg/mL-1000 pg/mL
靈敏度:
3.9 pg/mL
反應(yīng)時(shí)間:
1-5h
樣本體積:
50-100ul
檢測(cè)波長:
450 nm
研究領(lǐng)域:
Signal Transduction
測(cè)定原理:
quantitative
測(cè)定方法:
Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human BMP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
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1:1 | Average % | 94 |
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Range % | 89-97 |
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1:2 | Average % | 103 |
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Range % | 95-107 |
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1:4 | Average % | 92 |
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Range % | 82-94 |
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1:8 | Average % | 95 |
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Range % | 89-99 |
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回收率:
The recovery of human BMP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range |
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Serum (n=5) | 94 | 90-98 |
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EDTA plasma (n=4) | 86 |
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注意事項(xiàng):
1、試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存
2、濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果
3、各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣
4、請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(xnx5)
5、封板膜只限一次性使用,以避免交叉污染
6、底物請(qǐng)避光保存。
7、嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn)
8、所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理
9、.本試劑不同批號(hào)組分不得混用。
艾普蒂生物 品牌保障:
1、質(zhì)量保證: 產(chǎn)品質(zhì)量穩(wěn)定,每批次產(chǎn)品必須通過QA、QC檢測(cè)才可出庫;
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4、售后無憂:產(chǎn)品質(zhì)量問題包退包換
艾普蒂ELISA試劑盒的優(yōu)勢(shì):
1、高效、靈敏、特異的抗體;
2、穩(wěn)定的重復(fù)性和可靠性;
3、吸附性能好,空白值低,孔底透明度高的固相載體;
4、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型!
關(guān)鍵字: 人 (BMP-4) Elisa試劑盒;人骨成型蛋白4(BMP-4);Human BMP-4 ELISA Ki;人 (BMP-4) ELISA KIT;
湖北艾普蒂生物工程有限公司(簡(jiǎn)稱:艾普蒂)由在國內(nèi)科研試劑領(lǐng)域有著十幾年從業(yè)經(jīng)驗(yàn)的專業(yè)技術(shù)團(tuán)隊(duì)和企業(yè)管理團(tuán)隊(duì)組建而成,專門從事以抗體、蛋白、細(xì)胞、化學(xué)品為核心的試劑產(chǎn)品研發(fā)與銷售。
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