產(chǎn)品詳情

• 產(chǎn)品描述：

  This Human BMP2 ELISA Kit was designed for the quantitative measurement of Human BMP2 protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

• 別名：BDA2 ELISA Kit; BMP-2 ELISA Kit; BMP-2A ELISA Kit; Bmp2 ELISA Kit; BMP2_HUMAN ELISA Kit; BMP2A ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit

• 縮寫：BMP2

• Uniprot No.：

• 種屬：Homo sapiens (Human)

• 樣本類型：serum, urine, cell culture supernates, tissue homogenates

• 檢測(cè)范圍：62.5 pg/mL-4000 pg/mL

• 靈敏度：15.6 pg/mL

• 反應(yīng)時(shí)間：1-5h

• 樣本體積：50-100ul

• 檢測(cè)波長(zhǎng)：450 nm

• 研究領(lǐng)域：Signal Transduction
  

• 測(cè)定原理：quantitative

• 測(cè)定方法：Sandwich

• 精密度：

  Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

• 線性度：

  To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		90
  		Range %		80-95
  1:2		Average %		95
  		Range %		91-99
  1:4		Average %		97
  		Range %		92-106
  1:8		Average %		93
  		Range %		86-99

  回收率：

  The recovery of human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		95		89-100

1、試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存

2、濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果

3、各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣

4、請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值)，請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(xnx5)

5、封板膜只限一次性使用，以避免交叉污染

6、底物請(qǐng)避光保存。

7、嚴(yán)格按照說明書的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn)

8、所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理

9、.本試劑不同批號(hào)組分不得混用。

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