基因符號(hào): BRAF, BRAF1, RAFB1
Western blot analysis of recombinant BRAF proteins.
Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199).免疫熒光:
Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF(V600A) plasmid, pCDNA3-GFP-BRAF (V600D) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid pCDNA3-GFP-BRAF (V600M) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid, then fixed and stained with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199).
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