中文名稱
英文名字
Mouse Anti Streptavidin
供應(yīng)商
Prospec
產(chǎn)品貨號(hào)
ANT-345
背景資料
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
應(yīng)用類型
ELISA, Western blot
免疫原
Anti Streptavidin mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant Streptavidin amino acids 25-183 purified from E. coli and corresponding to the below amino acid sequence: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNNYRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ.
來源宿主
小鼠
反應(yīng)性
待更新
保存建議
4℃大約保存1個(gè)月, 為長期保存, 推薦保存于-20℃。 請避免反復(fù)凍融。
其他
ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司,具有20多年的生產(chǎn)經(jīng)驗(yàn),專注于蛋白(重組及合成)生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括:干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。n領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白,價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白,而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液,ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽,這就對(duì)工藝提出了更高的要求。同時(shí),ProSpec-Tany絕大部分產(chǎn)品為凍干粉,因此易于運(yùn)輸和保存。
湖北艾普蒂生物工程有限公司
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