中文名稱(chēng)

同源重組檢測(cè)試劑盒

英文名字

Homologous Recombination Assay Kit

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號(hào)

NGB-35600

背景資料

同源重組檢測(cè)試劑盒產(chǎn)品的背景資料詳情查看Norgen Biotek品牌提供的產(chǎn)品說(shuō)明書(shū)

產(chǎn)品描述

Norgen’s Homologous Recombination Assay Kit provides a rapid and sensitive tool for measuring the efficiency of homologous recombination in both bacterial and mammalian cells. The kit consists of 5 components: (1) dl-1 plasmid, (2) dl-2 plasmid, (3) positive control plasmid, (4) assay primers mixture, and (5) universal primers mixture. The assay is based on the co-transformation of the two dl plasmids into bacterial cells followed by the isolation of plasmid DNA and quantification of the homologous recombination efficiency through qPCR. In addition, PCR can be used for qualitative analysis of the recombination efficiency. The primer mixture provided with the kit is designed to amplify only the homologous recombination product but not the dl plasmids (dl-1 or dl-2). The two plasmids can be co-transfected into mammalian cells, then the DNA can be isolated and the homologous recombination efficiency can be assayed by qPCR (or PCR). In addition, the efficiency of homologous recombination can be assessed in bacteria by blue/white screening using X-gal. The provided positive control contains the functional LacZ sequence and can be used as a positive control for the plasmid DNA isolation efficiency (after transformation or transfection) or PCRs. The use of PCR-based homologous recombination assay enhances the sensitivity and eliminates the need for labor intensive screening by using traditional protein expression and staining techniques and is suitable for high throughput screening studies. The kit is designed to perform 200 transformations or 24 transfections.

產(chǎn)品特點(diǎn)

Rapid and sensitive tool for measuring the efficiency of homologous recombination; For any cell types including prokaryotes and eukaryotes; All primers included for quantification of the homologous recombination efficiency through qPCR.

保存建議

所有溶液應(yīng)密封保存，在-20℃或更低的溫度下保存長(zhǎng)達(dá)1年。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專(zhuān)利的碳化硅膜純化技術(shù)和無(wú)苯酚專(zhuān)利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國(guó)家研究委員會(huì)頒發(fā)的科技創(chuàng)新獎(jiǎng)，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測(cè)機(jī)分子診斷領(lǐng)域的研發(fā)能力）認(rèn)證的生物科技公司。 目前該公司的產(chǎn)品多達(dá)700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標(biāo)本類(lèi)型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過(guò)濾裝置。