中文名稱

內(nèi)涵體提取試劑盒

英文名字

ProteoSpin Inclusion Body Isolation Micro Kit

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號

NGB-10300

背景資料

Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these sub-cellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time consuming and not cost effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.

產(chǎn)品描述

This kit provides everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.

產(chǎn)品特點

The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 μg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

保存建議

Cell Lysis Reagent和IB Solubilization Reagent儲存在4°C。所有其他未開封的溶液應(yīng)在室溫下儲存。一旦打開，溶液應(yīng)儲存在4°C，除了堿性和酸性結(jié)合緩沖液，應(yīng)在室溫下儲存。具體保存情況收到產(chǎn)品后請按說明書存放。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專利的碳化硅膜純化技術(shù)和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測機分子診斷領(lǐng)域的研發(fā)能力）認(rèn)證的生物科技公司。 目前該公司的產(chǎn)品多達(dá)700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標(biāo)本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。