磷酸組氨酸性磷酸酶1(PHPT1)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Phosphohistidine Phosphatase 1
Gene Names: PHPT1, PHP14
Size: 100μg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Exhibits phosphohistidine phosphatase activity.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm
Tissue Specificity: Specificity: Specificity: Specificity: Expressed abundantly in heart and skeletal muscle.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Tyr125 (Accession # Q9NRX4), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 15.4 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 15.4 kDa
[PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage.
Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGSEF-MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV
SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
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關(guān)鍵字： PHP14;Phosphohistidine;Sex-Regulated;磷酸組氨酸性磷酸酶1(PHPT1)重組蛋;
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上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。