內(nèi)皮細胞粘附分子(ESAM)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Endothelial Cell Adhesion Molecule
Synonyms Synonyms Synonyms Synonyms: ESAM
Species: Species: Species: Species: Human
Size: 100μg
Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell junction, adherens junction. Cell junction, tight junction. Cell
membrane; Single-pass type I membrane protein.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Leu31~Val390 (Accession # Q96AP7), with N-terminal His-Tag.
Grade & Purity: Purity: Purity: Purity: >95%, 40 kDa as determined by SDS-PAGE reducing conditions.
Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 39.5 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-tag, its sequence is listed below.
MGHHHHHHSGSEF-LQLHLPANRL QAVEGGEVVL PAWYTLHGEV SSSQPWEVPF VMWFFKQKEK EDQVLSYING
VTTSKPGVSL VYSMPSRNLS LRLEGLQEKD SGPYSCSVNV QDKQGKSRGH SIKTLELNVL VPPAPPSCRL QGVPHVGANV
TLSCQSPRSK PAVQYQWDRQ LPSFQTFFAP ALDVIRGSLS LTNLSSSMAG VYVCKAHNEV GTAQCNVTLE VSTGPGAAVV
AGAVVGTLVG LGLLAGLVLL YHRRGKALEE PANDIKEDAI APRTLPWPKS SDTISKNGTL SSVTSARALR PPHGPPRPGA
LTPTPSLSSQ ALPSPRLPTT DGAHPQPISP IPGGVSSSGL SRMGAVPVMV PAQSQAGSLV
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關(guān)鍵字： Recombinant;Endothelial;ESAM;內(nèi)皮細胞粘附分子(ESAM)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠大； 更是一家專注于生命科學與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學，包括分子生物學、細胞生物學、免疫學試劑、重組蛋白，抗體，實驗技術(shù)服務(wù)等多個應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細胞系，原代細胞，常用實驗試劑，染色液等科研產(chǎn)品。