中文名稱

ELIPA 驅(qū)動蛋白ATP酶檢測試劑盒（酶聯(lián)無機磷酸鹽測定）

英文名字

Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM)

供應商

Cytoskeleton

產(chǎn)品貨號

BK060

產(chǎn)品報價

￥12484.368/KIT96assays

產(chǎn)品說明書

點擊查看

購買方式

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產(chǎn)品新聞

背景資料

該ELIPA 驅(qū)動蛋白ATP酶檢測試劑盒（酶聯(lián)無機磷酸鹽測定）產(chǎn)品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立專業(yè)技術(shù)支持團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務，在意您的成功！

產(chǎn)品描述

收到該ELIPA 驅(qū)動蛋白ATP酶檢測試劑盒（酶聯(lián)無機磷酸鹽測定）Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM)產(chǎn)品后，查看廠家提供的產(chǎn)品說明書詳細介紹。

產(chǎn)品特點

Eukaryotic kinesin motor proteins orchestrate a wide range of kinetic events within a cell. They have been shown to move cargoes, such as chromosomes and vesicles, along microtubule tracks (1). They also play a major role in the organization of cytoskeletal architecture as evidenced in the establishment of the microtubule spindle during mitosis (2). Kinesins operate by utilizing the energy of ATP hydrolysis to move along their microtubule (MT) substrates. Once a kinesin motor binds to its MT track, the ATPase rate of the motor is often enhanced several hundred to several thousand fold (3). MT activated kinesin ATPase is a major parameter in motor function and serves as a powerful method to monitor and study kinesin activity under various experimental conditions. As part of its Cytoskeleton Motor Werks (CMW) line of research reagents, Cytoskeleton, Inc. has developed the Kinesin ELIPA? (Enzyme Linked Inorganic Phosphate Assay) Biochem Kit. The assay is an adaptation of a method originally described by Webb for the measurement of glycerol kinase plus D-glyceraldehyde ATPase activity and for actin activated myosin ATPase (4). The assay is based upon an absorbance shift (330 - 360 nm) that occurs when 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methyl purine in the presence of inorganic phosphate (Pi). The reaction is catalyzed by purine nucleoside phosphorylase (PNP). One molecule of inorganic phosphate will yield one molecule of 2-amino-6-mercapto-7-methyl purine in an essentially irreversible reaction (5). Thus, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the kinesin ATPase reaction.

保存建議

廠家推薦常溫運輸，收到產(chǎn)品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號轉(zhuǎn)導、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細胞骨架相關(guān)研究見長，既能滿足于樣品較少的科學研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務。