凋亡誘導(dǎo)因子(AIF)重組蛋白
[ PROPERTIES ]
Residues: Gly103~Lys322
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O95831
Host: E. coli
Subcellular Location: Mitochondrion
intermembrane space. Mitochondrion inner
membrane. Nucleus. Cytoplasm, perinuclear
region.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.5
Predicted Molecular Mass: 27.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GLTPEQKQ KKAALSASEG EEVPQDKAPS HVPFLLIGGG TAAFAAARSI RARDPGARVL
IVSEDPELPY MRPPLSKELW FSDDPNVTKT LRFKQWNGKE RSIYFQPPSF YVSAQDLPHI
ENGGVAVLTG KKVVQLDVRD NMVKLNDGSQ ITYEKCLIAT GGTPRSLSAI DRAGAEVKSR
TTLFRKIGDF RSLEKISREV KSITIIGGGF LGSELACALG RK
[ REFERENCES ]
1.Susin S.A., et al. (1999) Nature 397:441-446
2. Delettre C., et al. (2006) J. Biol. Chem. 281:6413-6427.
3. Delettre C., et al. (2006) J. Biol. Chem. 281:18507-18518.
4. Otera H., et al. (2005) EMBO J. 24:1375-1386.
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關(guān)鍵字： PDCD8;Programmed;Apoptosis;凋亡誘導(dǎo)因子(AIF)重組蛋白;
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