輔肌動蛋白α2(ACTN2)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Actinin Alpha 2
Gene Names: ACTN2
Size: 100μg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: F-actin cross-linking protein which is thought to anchor actin to a variety of
intracellular structures. This is a bundling protein.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm; myofibril; sarcomere; Z line. Note: Colocalizes with
MYOZ1 and FLNC at the Z-lines of skeletal muscle.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~His254 (Accession # Q9JI91), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 31 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 31 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short time storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage.
Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGSEF-MNQIEPGVQY NYVYDEDEYM IQEEEWDRDL LLDPAWEKQQ RKTFTAWCNS
HLRKAGTQIE NIEEDFRNGL KLMLLLEVIS GERLPKPDRG KMRFHKIANV NKALDYIASK GVKLVSIGAE
EIVDGNVKMT LGMIWTIILR FAIQDISVEE TSAKEGLLLW CQRKTAPYRN VNIQNFHTSW KDGLGLCALI
HRHRPDLIDY SKLNKDDPIG NINLAMEIAE KHLDIPKMLD AEDIVNTPKP DERAIMTYVS CFYH
關(guān)鍵字: Alpha;F-actin;ACTN2;輔肌動蛋白α2(ACTN2)重組蛋白;
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