雙解絲蛋白1(TWF1)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Twinfilin 1
Gene Names: TWF1
Size: 100μg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Actin-binding protein involved in motile and morphological processes. Inhibits
actin polymerization, likely by sequestering G-actin. By capping the barbed ends of
filaments, it also regulates motility. Seems to play an important role in clathrin-mediated
endocytosis and distribution of endocytic organelles
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cytoplasm ? cytoskeleton
Tissue Specificity: Specificity: Specificity: Specificity: Expressed at high levels in the colon, testis, ovary, prostate and
lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Ser252 (Accession # Q12792), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 30.26 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 30.26 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL
LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE VFGTVKEDVS
LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF PISREAFQAL EKLNNRQLNY
VQLEIDIKNE IIILANTTNT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL ES
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Beeler JF, et al.(1994) Mol Cell Biol 14 (2): 982-988.
2. Rush J., et al. (2005) Nat. Biotechnol. 23:94-101. 3. Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162. 4. Palmgren S, et al.(2002) J. Cell. Sci. 115 (Pt 5): 881-886.
5. Vartiainen MK, et al.(2003) J. Biol. Chem. 278 (36): 34347-34355.
6. Moseley,J.B. et al. (2006) J Cell Sci. 119 (Pt 8):1547-1557.
7. Hassel S, et al. (2004) Proteomics 4 (5): 1346-1358.
8. Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988.
關(guān)鍵字： A6;PTK9;Actin;雙解絲蛋白1(TWF1)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊(cè)地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號(hào)。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競(jìng)爭(zhēng)力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測(cè)試劑盒、PCR檢測(cè)試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。