Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

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純度/分析方法:

別名：

• 雙苯甲亞胺 H 33258 (0.2mL×5) (1mg/mL水溶液) [用于細(xì)胞染色]
• 2'-(4-羥苯基)-5-(4-甲基-1-哌啶基)-2,5'-聯(lián)苯并咪唑三鹽酸鹽 (0.2mL×5) (1mg/mL水溶液)
• 4-[5-(4-甲基-1-哌啶基)-1H,1'H-[2,5'-聯(lián)苯并咪唑]-2'-基]苯酚三鹽酸鹽 (0.2mL×5) (1mg/mL水溶液)
• 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride (0.2mL×5) (1mg/mL in Water)
• 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-[2,5'-bibenzimidazol]-2'-yl]phenol Trihydrochloride (0.2mL×5) (1mg/mL in Water)

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Product Information
Volume : 0.2 mL x 5 vial
Concentration : 1.0 mg/mL

Description
Bisbenzimide H 33258 is a fluorescent stain for labeling DNA.
DNA-bound Bisbenzimide H 33258 is readily excited with UV and emits fluorescence in the blue region of the visible light spectrum (Excitation : 355 nm / Emission : 465 nm).

Description for Use
Fluorescence Staining of Cell Nucleus
Fix and permeabilize cells as desired.
Dilute Bisbenzimide H 33258 solution 1/1000* with PBS or an appropriate buffer.
Incubate with cells at room temperature for 5-20 minutes**.
*Typical working concentration used in most applications is 0.1μg/mL-10μg/mL.
Incubate with cells at room temperature for 5-30 minutes**.
** Extend Incubate time as desired.
Mount sample with an appropriate medium and proceed to imaging.

Storage
Store at －20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

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