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  2. Anti-infection
  3. Bacterial
  4. Amifloxacin

Amifloxacin  (Synonyms: 氨氟沙星; Win49375)

目錄號: HY-U00221 純度: 99.23%
COA 產品使用指南 技術支持

Amifloxacin (Win49375) 是一種合成的喹諾酮類抗菌劑。

MCE 的所有產品僅用作科學研究或藥證申報，我們不為任何個人用途提供產品和服務

Amifloxacin Chemical Structure

Amifloxacin Chemical Structure

CAS No. : 86393-37-5

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規(guī)格 價格 是否有貨 數量
1 mg ￥1500
In-stock
5 mg ￥3750
In-stock
10 mg ￥6000
In-stock
25 mg 現貨 詢價
50 mg   詢價  
100 mg   詢價  

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  • 生物活性

  • 實驗參考方法

  • 純度 & 產品資料

  • 參考文獻

生物活性

Amifloxacin (Win49375) is a synthetic antibacterial agent of the quinolone class.

IC50 & Target

Antibacterial[1]
體外研究
(In Vitro)

Amifloxacin (WIN 49375) is active in vitro against Pseudomonas aeruginosa isolates and shows moderate activity against Staphylococcus aureus, with MICs of less than or equal to 2 μg/mL.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內研究
(In Vivo)

Amifloxacin (WIN 49375) is highly active by the oral route, with 50% effective doses within two- to threefold of those obtained with parenteral medication. The effectiveness of Amifloxacin with various routes of medication is demonstrated with these experimental infections. When mice infected intraperitoneally with E. coli Vogel are medicated at 0.5-h postinfection subcutaneously, intravenously, or orally the ED50s for Amifloxacin are 0.6, 0.8, and 1.0 mg/kg, respectively[1]. Blood radioactivity peaks at 0.5 h after oral administration of [14C]Amifloxacin mesylate to rats at 20 mg/kg and is equivalent to 7.1±0.26 μg of Amifloxacin per mL. From 0.75 to 4 h, blood radioactivity decreases rapidly from 7.0±0.25 μg/mL to 1.2±0.12 μg/mL. Between 8 and 48 h the rate of decline in blood radioactivity slows and is more complex. At 48 h, the blood radioactivity is equivalent to 0.14±0.02 μg/mL. Blood levels of radioactivity after i.v. administration of [14C]Amifloxacin mesylate to rats at 20 mg/kg decrease from 29.1±0.85 μg/mL at 1.0 min to 14.4±0.52 μg/mL at 10 min. From 0.25 to 4 h blood radioactivity decreases from 13.0±0.42 μg/mL to 0.97±0.09 μg/mL in a log-linear manner. The rate of elimination from 4 to 24 h is slower and more complex. At 24 h, blood radioactivity is equivalent to 0.12±0.01 ug/mL[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

334.35

Formula

C16H19FN4O3
CAS 號
性狀

固體

顏色

White to light yellow

中文名稱

氨氟沙星；氨氟哌酸
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數據
細胞實驗： 

H2O 中的溶解度 : 50 mg/mL (149.54 mM; 超聲助溶; 堿性條件溶解 (NaOH 調節(jié)，pH≈11))

配制儲備液
濃度 溶劑體積 質量 1 mg 5 mg 10 mg
1 mM 2.9909 mL 14.9544 mL 29.9088 mL
5 mM 0.5982 mL 2.9909 mL 5.9818 mL
查看完整儲備液配制表

* 請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結果
工作液所需濃度 : mg/mL
該產品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數據。
您所需的儲備液濃度超過該產品的實測溶解度，如有需要，請與 MCE 中國技術支持聯(lián)系。
純度 & 產品資料
參考文獻
Animal Administration
[2]

Rats[2]
In each study, groups of eight Sprague-Dawley rats (four per sex) are dosed either orally bygavage or intravenously via the jugular vein with [14C] Amifloxacin mesylate at 20 mg/kg. All doses are prepared as solutions in distilled water. Orally medicated animals are fasted overnight before dosing. Rats in absorption studies are held in restrainers while serial blood samples are collected from the tail vein into 50 μL heparinized capillary tubes. Rats in the excretion and distribution studies are maintained in individual metabolism cages. In the distribution study, groups of rats are killed at either 0.5 or 6.0 h, blood is collected from the abdominal aorta, and tissues and organs are dissected.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

Amifloxacin 相關分類

完整儲備液配制表

* 請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.9909 mL 14.9544 mL 29.9088 mL 74.7719 mL
5 mM 0.5982 mL 2.9909 mL 5.9818 mL 14.9544 mL
10 mM 0.2991 mL 1.4954 mL 2.9909 mL 7.4772 mL
15 mM 0.1994 mL 0.9970 mL 1.9939 mL 4.9848 mL
20 mM 0.1495 mL 0.7477 mL 1.4954 mL 3.7386 mL
25 mM 0.1196 mL 0.5982 mL 1.1964 mL 2.9909 mL
30 mM 0.0997 mL 0.4985 mL 0.9970 mL 2.4924 mL
40 mM 0.0748 mL 0.3739 mL 0.7477 mL 1.8693 mL
50 mM 0.0598 mL 0.2991 mL 0.5982 mL 1.4954 mL
60 mM 0.0498 mL 0.2492 mL 0.4985 mL 1.2462 mL
80 mM 0.0374 mL 0.1869 mL 0.3739 mL 0.9346 mL
100 mM 0.0299 mL 0.1495 mL 0.2991 mL 0.7477 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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