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  2. Anti-infection Apoptosis
  3. Fungal Apoptosis
  4. Lawsone

Lawsone  (Synonyms: 2-羥基-1,4-萘醌)

目錄號: HY-N2493 純度: 99.36%
COA 產(chǎn)品使用指南

Lawsone 是一種具有口服活性的萘醌染料，可從 Lawsonia inermis 的葉片中分離得到。Lawsone 可誘導細胞凋亡 (apoptosis)。Lawsone 有抗菌、抗腫瘤和抗氧化活性。Lawsone 可用于抗腫瘤藥物研究。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Lawsone Chemical Structure

Lawsone Chemical Structure

CAS No. : 83-72-7

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     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥610
In-stock
500 mg ￥550
In-stock
1 g   詢價  
5 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Lawsone:

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Lawsone is an orally active naphthoquinone dye that can be isolated from the leaves of Lawsonia inermis. Lawsone can induce apoptosis. Lawsone has antibacterial, antitumor and antioxidant activities. Lawsone can be used in anti-tumor drug research[1][2][3][4][5].

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
58 μM
Compound: 17
Growth inhibition of human A549 cells by MTT assay
Growth inhibition of human A549 cells by MTT assay
[PMID: 24374273]
HeLa IC50
> 100 μM
Compound: 1c
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 24913712]
Hepatocyte CC50
> 500 μM
Compound: Lawsone
Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay
Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay
[PMID: 29289882]
HFF-1 IC50
> 50 μM
Compound: 7
Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
[PMID: 34438124]
Hs 683 IC50
31 μM
Compound: 17
Growth inhibition of human Hs683 cells by MTT assay
Growth inhibition of human Hs683 cells by MTT assay
[PMID: 24374273]
LoVo IC50
> 100 μM
Compound: 17
Growth inhibition of human LoVo cells by MTT assay
Growth inhibition of human LoVo cells by MTT assay
[PMID: 24374273]
MCF7 IC50
> 50 μM
Compound: 1
Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
Cytotoxicity against ER-positive human MCF7 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
[PMID: 31810778]
MDA-MB-231 IC50
> 50 μM
Compound: 1
Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
[PMID: 31810778]
PC-3 IC50
> 100 μM
Compound: 17
Growth inhibition of human PC3 cells by MTT assay
Growth inhibition of human PC3 cells by MTT assay
[PMID: 24374273]
SAS IC50
> 100 μM
Compound: 1c
Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 24913712]
SK-BR-3 IC50
> 50 μM
Compound: 1
Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
Cytotoxicity against HER2-positive human SKBR3 cells assessed as redution in cell viability incubated for 72 hrs by SRB assay
[PMID: 31810778]
SK-MEL-28 IC50
> 100 μM
Compound: 17
Growth inhibition of human SK-MEL-28 cells by MTT assay
Growth inhibition of human SK-MEL-28 cells by MTT assay
[PMID: 24374273]
U-373MG ATCC IC50
> 100 μM
Compound: 17
Growth inhibition of human U373 cells by MTT assay
Growth inhibition of human U373 cells by MTT assay
[PMID: 24374273]
Vero CC50
213.74 μM
Compound: Lawsone
Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay
Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay
[PMID: 29289882]
體外研究
(In Vitro)

Lawsone (125, 250, 500 μM, 18 h) 劑量依賴性的抑制大腸桿菌的生長[3]。
Lawsone (0.5, 1, 1.5, 2 mg/mL, 48 h) 在人 DLD-1 細胞中通過降低 NF-κB 活性導致細胞周期蛋白 B1 和 cdk1 的表達水平受到抑制而抑制細胞增殖[4]。
Lawsone (10, 20, 40, 80, 160 mg/mL, 24 h) 在 SKOV-3 卵巢癌細胞中具有抗增殖作用，通過抑制 Bcl-2 誘導細胞凋亡[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[4]

Cell Line: DLD-1, MRC-5
Concentration: 0.5, 1, 1.5, 2 mg/mL
Incubation Time: 24-96 h
Result: Demonstrated significant suppression at concentration 2 mg/ml on DLD-1 cells and exhibited no suppression on normal fibroblast MRC-5 cells.

Western Blot Analysis[4]

Cell Line: DLD-1
Concentration: 0.5, 1, 1.5, 2 mg/mL
Incubation Time: 48 h
Result: Decreased the level of both proteins cyclin B1 and cdk1.
Exhibited a dose-dependent effect on translocation of p65 of cytosol to the nucleus.
體內(nèi)研究
(In Vivo)

Lawsone (62.5, 125, 250, 500, 750 μmol/kg/day, 口服) 在大鼠中可誘導與紅細胞氧化損傷相關(guān)的溶血反應[2]。
Lawsone (200 mg/mL, 口服, 持續(xù) 8 周) 對大鼠結(jié)腸癌有明顯的抑制作用[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female rat model (10-11- week-old)[2]
Dosage: 62.5, 125, 250, 500, 750 μmol/kg/day
Administration: p.o.
Result: Increased the levels of ALT, AST, GLDH, LDH, HBDH, urea and creatinine at a dose of 500 or 750 μmol/kg/day.
Animal Model: Rat model of colon cancer[4]
Dosage: 200 mg/mL
Administration: p.o.
Result: Reduced the tumor diameters and decreased multiplicity of these adenomas.
Decreased the PCNA staining index
分子量

174.16

Formula

C10H6O3
CAS 號
性狀

固體

顏色

Light yellow to yellow

結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 100 mg/mL (574.20 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 5.7420 mL 28.7101 mL 57.4201 mL
5 mM 1.1484 mL 5.7420 mL 11.4840 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.36 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.36 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.36%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.7420 mL 28.7101 mL 57.4201 mL 143.5503 mL
5 mM 1.1484 mL 5.7420 mL 11.4840 mL 28.7101 mL
10 mM 0.5742 mL 2.8710 mL 5.7420 mL 14.3550 mL
15 mM 0.3828 mL 1.9140 mL 3.8280 mL 9.5700 mL
20 mM 0.2871 mL 1.4355 mL 2.8710 mL 7.1775 mL
25 mM 0.2297 mL 1.1484 mL 2.2968 mL 5.7420 mL
30 mM 0.1914 mL 0.9570 mL 1.9140 mL 4.7850 mL
40 mM 0.1436 mL 0.7178 mL 1.4355 mL 3.5888 mL
50 mM 0.1148 mL 0.5742 mL 1.1484 mL 2.8710 mL
60 mM 0.0957 mL 0.4785 mL 0.9570 mL 2.3925 mL
80 mM 0.0718 mL 0.3589 mL 0.7178 mL 1.7944 mL
100 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4355 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Lawsone
目錄號：
HY-N2493
需求量：