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  2. Cell Cycle/DNA Damage Autophagy
  3. CDK Autophagy
  4. Cucurbitacin E

Cucurbitacin E  (Synonyms: 葫蘆素 E; α-Elaterin; α-Elaterine)

目錄號(hào): HY-N0417 純度: 99.92%
COA 產(chǎn)品使用指南 技術(shù)支持

Cucurbitacin E 是來(lái)自葫蘆科的一種天然化合物。Cucurbitacin E 顯著抑制 cyclin B1/CDC2 復(fù)合物的活性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Cucurbitacin E Chemical Structure

Cucurbitacin E Chemical Structure

CAS No. : 18444-66-1

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規(guī)格 價(jià)格 是否有貨 數(shù)量
1 mg ￥548
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5 mg ￥1200
In-stock
10 mg ￥1900
In-stock
25 mg ￥3338
In-stock
50 mg ￥4863
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100 mg 現(xiàn)貨 詢(xún)價(jià)
200 mg   詢(xún)價(jià)  
500 mg   詢(xún)價(jià)  

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Customer Review

Other Forms of Cucurbitacin E:

    Cucurbitacin E purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2020 May 20;5(1):56.  [Abstract]

    Western Blot is performed to determine the protein levels of cyclin B1, Cdk1, and p-Drp1 Ser616. LMP1-positive cells are treated with or without cucurbitacin E (10?μM) for 24?h.

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Cucurbitacin E is a natural compound which from Cucurbitaceae plants. Cucurbitacin E significantly suppresses the activity of the cyclin B1/CDC2 complex.

    IC50 & Target[1]

    cyclin B1/CDC2

     

    Autophagy

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    HeLa IC50
    0.1 μM
    Compound: 12
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
    		[PMID: 21459003]
    HepG2 IC50
    15.25 μM
    Compound: 12
    Cytotoxicity against human HepG2 cells after 24 hrs assessed as inhibition of cell viability by MTT assay
    Cytotoxicity against human HepG2 cells after 24 hrs assessed as inhibition of cell viability by MTT assay
    		[PMID: 21459003]
    HepG2 EC50
    3.2 μM
    Compound: 12
    Hepatoprotective activity in human HepG2 cells assessed as inhibition of CCl4-induced toxicity after 24 hrs by MTT assay
    Hepatoprotective activity in human HepG2 cells assessed as inhibition of CCl4-induced toxicity after 24 hrs by MTT assay
    		[PMID: 21459003]
    HL-60 IC50
    18 nM
    Compound: 1
    Cytotoxicity against human HL60 cells after 72 hrs by WST-8 assay
    Cytotoxicity against human HL60 cells after 72 hrs by WST-8 assay
    		[PMID: 20347305]
    HSC-T6 EC50
    0.04 μM
    Compound: 12
    Antiproliferative activity against serum-stimulated rat HSC-T6 cells after 24 hrs by MTT assay
    Antiproliferative activity against serum-stimulated rat HSC-T6 cells after 24 hrs by MTT assay
    		[PMID: 21459003]
    HSC-T6 IC50
    2.03 μM
    Compound: 12
    Cytotoxicity against rat HSC-T6 cell after 24 hrs by MTT assay
    Cytotoxicity against rat HSC-T6 cell after 24 hrs by MTT assay
    		[PMID: 21459003]
    HT-1080 IC50
    40 nM
    Compound: 1
    Cytotoxicity against human HT1080 cells after 72 hrs by WST-8 assay
    Cytotoxicity against human HT1080 cells after 72 hrs by WST-8 assay
    		[PMID: 20347305]
    JY IC50
    0.18 μM
    Compound: 1
    Inhibition of LFA1 expressed in human JY cells interaction with ICAM1-IG expressed in human HeLa cell monolayer after 45 mins by cell adhesion assay
    Inhibition of LFA1 expressed in human JY cells interaction with ICAM1-IG expressed in human HeLa cell monolayer after 45 mins by cell adhesion assay
    		[PMID: 7852999]
    MCF7 IC50
    0.055 μM
    Compound: 8
    Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay
    Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTS/PMS assay
    		[PMID: 25756299]
    U-937 IC50
    16 nM
    Compound: 1
    Cytotoxicity against human U937 cells after 72 hrs by WST-8 assay
    Cytotoxicity against human U937 cells after 72 hrs by WST-8 assay
    		[PMID: 20347305]
    體外研究
    (In Vitro)

    為了探索Cucurbitacin E (CuE) 對(duì)結(jié)直腸癌 (CRC) 細(xì)胞的抗腫瘤活性，啟動(dòng)了一項(xiàng)體外研究，其中每個(gè) CRC 細(xì)胞系暴露于增加劑量的Cucurbitacin E (0、2.5、5 和 7.5 μM) 在 24 小時(shí)內(nèi)。然后使用 MTT 方法測(cè)量Cucurbitacin E 處理的癌細(xì)胞的增殖。Cucurbitacin E 顯示可誘導(dǎo)原發(fā)性結(jié)腸癌細(xì)胞的形態(tài)學(xué)變化。顯微鏡觀察表明，在接觸Cucurbitacin E (5 μM) 6 至 24 小時(shí)后，原發(fā)性結(jié)腸癌細(xì)胞的形態(tài)發(fā)生了顯著變化。Cucurbitacin E 通過(guò) GADD45γ 基因表達(dá)和阻斷原代 CRC 細(xì)胞中的細(xì)胞周期蛋白 B1/CDC2 復(fù)合物使細(xì)胞周期停滯在 G2/M 期，從而抑制腫瘤生長(zhǎng)[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Cucurbitacin E (0.5mg/kg) 處理的 HFD-MetS 小鼠的體重顯著降低，脂肪墊重量減少[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    556.69

    Formula

    C32H44O8
    CAS 號(hào)
    性狀

    固體

    顏色

    White to off-white

    中文名稱(chēng)

    葫蘆素 E
    結(jié)構(gòu)分類(lèi)
    初始來(lái)源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 50 mg/mL (89.82 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.7963 mL 8.9817 mL 17.9633 mL
    5 mM 0.3593 mL 1.7963 mL 3.5927 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (4.49 mM); 懸濁液; 超聲助溶

      此方案可獲得 2.5 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.49 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個(gè)月以上的動(dòng)物實(shí)驗(yàn)酌情使用。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL玉米油中，混合均勻。

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
    請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過(guò) 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過(guò)半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
    請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.92%

    參考文獻(xiàn)
    Cell Assay
    [1]

    The colorectal cancer (CRC) cells are seeded into 96-well culture plates at 5000 cells/well. The cells are treated with 0, 2.5, 5, and 7.5?μM Cucurbitacin E for 1-3 days. MTT dye (1?mg/mL) is added to each well for at least 4?h of treatment. The reaction is stopped by the addition of DMSO, and optical density is measured at 540?nm on a multi-well plate reader. Background absorbance of the medium in the absence of cells is subtracted. All samples are assayed in triplicate, and the mean for each experiment is calculated. Results are expressed as a percentage of control, which is considered as 100%. Each assay is carried out in triplicate, and the results are expressed as the mean[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    C57BL/6 male mice are used. The mice are designated as metabolic syndrome mice (HFD-MetS-mice). Briefly, the mice are randomly assigned into two groups according to their diet for 8 weeks (n = 10-12): high fat diet group (HFD) (60% fat, 20% carbohydrate, 20% protein) or the matched low fat, standard diet group (SD) (10% fat, 70% carbohydrate, 20% protein). After eight weeks on high fat diet, the mice with significant obese phenotype and fasting blood glucose levels ≥126 mg/dL are considered MetS mice. The MetS mice are continued on the HFD throughout the study. The MetS mice are then randomly divided into three additional groups, according to the treatment administered by oral gavage for 10 weeks (n=10-12): a low dose 0.25 mg/kg/day of Cucurbitacin E designated as HFD+Cucurbitacin E (L) or high dose 0.5 mg/kg/day of Cucurbitacin E, designated as HFD+Cucurbitacin E (H) or 50 mg/kg/day Orlistat (HFD+Orlistat). Animals on SD are administered 0.5% CMC by oral gavage[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7963 mL 8.9817 mL 17.9633 mL 44.9083 mL
    5 mM 0.3593 mL 1.7963 mL 3.5927 mL 8.9817 mL
    10 mM 0.1796 mL 0.8982 mL 1.7963 mL 4.4908 mL
    15 mM 0.1198 mL 0.5988 mL 1.1976 mL 2.9939 mL
    20 mM 0.0898 mL 0.4491 mL 0.8982 mL 2.2454 mL
    25 mM 0.0719 mL 0.3593 mL 0.7185 mL 1.7963 mL
    30 mM 0.0599 mL 0.2994 mL 0.5988 mL 1.4969 mL
    40 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
    50 mM 0.0359 mL 0.1796 mL 0.3593 mL 0.8982 mL
    60 mM 0.0299 mL 0.1497 mL 0.2994 mL 0.7485 mL
    80 mM 0.0225 mL 0.1123 mL 0.2245 mL 0.5614 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱(chēng)：
    Cucurbitacin E
    目錄號(hào)：
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