Scopoletin (Synonyms: 東莨菪內(nèi)酯; Gelseminic acid; Chrysatropic acid)

目錄號: HY-N0342 純度: 99.89%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計(jì)算器

Scopoletin 是一種乙酰膽堿酯酶 (AChE) 抑制劑。

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Scopoletin Chemical Structure

CAS No. : 92-61-5

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可免費(fèi)申領(lǐng)三個不同產(chǎn)品的試用裝。

3. 試用裝只面向終端客戶。

規(guī)格	價(jià)格	是否有貨
10 mM * 1 mL in DMSO	￥770	In-stock
50 mg	￥700	In-stock
100 mg	￥990	In-stock
200 mg	￥1600	In-stock
500 mg		詢價(jià)
1 g		詢價(jià)

or 大包裝詢價(jià)

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Customer Review

Other Forms of Scopoletin:

Scopoletin (Standard) 詢價(jià)

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

•Phytopathology Research. 2024 Jan 2.
•Oxid Med Cell Longev. 2019 May 2;2019:2761041 [Abstract]

Scopoletin 相關(guān)產(chǎn)品

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AChE BChE

生物活性
實(shí)驗(yàn)參考方法
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

Scopoletin is an inhibitor of acetylcholinesterase (AChE).

IC₅₀ & Target

AChE

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A431 cells after 24 hrs by MTT assay Cytotoxicity against human A431 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
A549	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
BXPC-3	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay	[PMID: 23218780]
HCT-116	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 23218780]
HCT-116	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay Growth inhibition of human HCT116 cells after 48 hrs by MTT assay	[PMID: 27956344]
HCT-116	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
HeLa	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
HepG2	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23218780]
HepG2	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
HL-60	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HL60 cells after 24 hrs by MTT assay Cytotoxicity against human HL60 cells after 24 hrs by MTT assay	[PMID: 23218780]
HL-60	IC₅₀	9.5 μM Compound: 3	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 15387667]
HT-1080	IC₅₀	12.5 μM Compound: 3	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 15387667]
HT-29	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
Huh-7	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human HuH7 cells after 48 hrs by MTT assay Growth inhibition of human HuH7 cells after 48 hrs by MTT assay	[PMID: 27956344]
HuT78	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay	[PMID: 23218780]
K562	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human K562 cells after 24 hrs by MTT assay Cytotoxicity against human K562 cells after 24 hrs by MTT assay	[PMID: 23218780]
L02	IC₅₀	> 25 μM Compound: Scopoletin	Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 32438251]
L6	IC₅₀	> 90 μM Compound: 4	Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry	[PMID: 21438586]
LoVo	IC₅₀	18.5 μM Compound: 3	Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
LoVo	IC₅₀	7.3 μM Compound: 3	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
Macrophage	IC₅₀	5 μM Compound: 4, scopoletin	Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method	[PMID: 8988605]
MCF-10A	IC₅₀	> 25 μM Compound: Scopoletin	Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MCF7	IC₅₀	> 25 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	IC₅₀	> 25 μM Compound: Scopoletin	Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
MCF7	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MDA-MB-231	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay	[PMID: 23218780]
MDA-MB-231	IC₅₀	> 100 μM Compound: 7, scopoletin	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MDA-MB-231	IC₅₀	> 25 μM Compound: Scopoletin	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MDA-MB-231	IC₅₀	> 30 μM Compound: 6, Scopoletin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
MDA-MB-435	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay	[PMID: 23218780]
MGC-803	IC₅₀	> 80 μM Compound: Scopoletin	Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MKN-45	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay	[PMID: 23218780]
PANC-1	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay	[PMID: 23218780]
PBMC	IC₅₀	> 100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PBMC after 24 hrs by MTT assay Cytotoxicity against human PBMC after 24 hrs by MTT assay	[PMID: 23218780]
PC-3	IC₅₀	72.19 μM Compound: Scopoletin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
Platelet	IC₅₀	> 50 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry	[PMID: 21106454]
Platelet	IC₅₀	41.74 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry	[PMID: 21106454]
Sf21	IC₅₀	> 100 μM Compound: 7	Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay	[PMID: 17378609]
SK-MEL-28	IC₅₀	3.43 μM Compound: 2	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	1.5 μM Compound: 2	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SW-620	IC₅₀	> 25 μM Compound: Scopoletin	Growth inhibition of human SW620 cells after 48 hrs by MTT assay Growth inhibition of human SW620 cells after 48 hrs by MTT assay	[PMID: 27956344]
U-937	CC₅₀	> 2000 μM Compound: 8	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	IC₅₀	151.5 μM Compound: 3	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]
U-937	CC₅₀	600.2 μM Compound: 3	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	IC₅₀	975.9 μM Compound: 8	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]

體外研究
(In Vitro)

Scopoletin (SCT) 被確定為乙酰膽堿酯酶 (AChE) 的推定抑制劑。Scopoletin 增強(qiáng) K⁺ 刺激的大鼠額葉皮質(zhì)突觸體 ACh 釋放，顯示出鐘形劑量效應(yīng)曲線 (E_max：4 μM)^[1]。Scopoletin 通過誘導(dǎo) PC3 細(xì)胞凋亡來抑制 PC3 增殖。Scopoletin抑制PC3、PAA (人肺癌細(xì)胞)、Hela 細(xì)胞增殖的 IC₅₀ 分別為 (157±25)、(154±51)、(294±100) mg/L。Scopoletin 誘導(dǎo)顯著的時(shí)間和濃度依賴性 PC3 細(xì)胞增殖抑制。Scopoletin 以濃度依賴性方式降低 PC3 細(xì)胞中的蛋白質(zhì)含量和酸性磷酸酶活性 (ACP) 水平。光鏡、熒光顯微鏡、透射電鏡觀察 Scopoletin 處理后的細(xì)胞，顯示出典型的細(xì)胞凋亡形態(tài)學(xué)變化。Scopoletin 0、100、200、400 mg/L 時(shí)細(xì)胞凋亡率分別為 0.3%、2.1%、9.3%、35%，東莨菪素處理后 G2 期細(xì)胞明顯減少^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Scopoletin (2 μg，icv) 增加 T 迷宮交替并改善東莨菪堿誘導(dǎo)的膽堿能缺陷小鼠的新物體識別。 Scopoletin 還可以減少 15-18 個月大的小鼠（2 mg/kg sc）的對象記憶中與年齡相關(guān)的缺陷。注射 2 μg Scopoletin 的小鼠表現(xiàn)出 71.3±2.5% 的交替率增加^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

192.17

Formula

C₁₀H₈O₄

CAS 號

92-61-5

性狀

固體

顏色

White to yellow

中文名稱

東莨菪內(nèi)酯；莨菪亭；東莨菪亭

結(jié)構(gòu)分類

初始來源

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

細(xì)胞實(shí)驗(yàn)：

DMSO 中的溶解度 : 62.5 mg/mL (325.23 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

*Scopoletin is usually formulated as a suspension.

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	5.2037 mL	26.0186 mL	52.0373 mL
5 mM	1.0407 mL	5.2037 mL	10.4075 mL
10 mM	0.5204 mL	2.6019 mL	5.2037 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時(shí)，請?jiān)?個月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個月內(nèi)使用。

摩爾計(jì)算器
稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實(shí)驗(yàn)：

請根據(jù)您的實(shí)驗(yàn)動物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.82 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (10.82 mM); 懸濁液; 超聲助溶

此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.82 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個月以上的動物實(shí)驗(yàn)酌情使用。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計(jì)算器

請輸入動物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實(shí)驗(yàn)過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計(jì)算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲備液濃度超過該產(chǎn)品的實(shí)測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.89%

選擇批次：

Data Sheet (651 KB) SDS (393 KB)

COA (286 KB) LCMS (94 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. Hornick A, et al. The coumarin Scopoletin potentiates acetylcholine release from synaptosomes, amplifies hippocampal long-term potentiation and ameliorates anticholinergic- and age-impaired memory. Neuroscience. 2011 Dec 1;197:280-92. [Content Brief]

[2]. Liu XL, et al. Effect of Scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001 Oct;22(10):929-33. [Content Brief]

Cell Assay ^[2]	PC3 cells (5×10⁷/L) 1 mL in exponential growth are seeded into four 24-well plates. The plates are incubated at 37°C in a humidified 5% CO₂ atmospbee. After 24h, Scopoletin 33, 66, 133, 266, and 533 mg/L are added to wells (3 wells for each concentration for each plate). For control cells (3 wells foreach plate),only DMEM was added. The plates are incubated continually. The viable cells are counted by hemocytometer every day in the frist 4 d by Trypan blue dye exclusion method^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] C56BL/6N male, 4-6-month-old and 16-18-month-old mice are used in the behavioral studies. The mice are housed in groups of four in cages at constant humidity (50-55%) and temperature (22±1°C) on a 12:12 h light/dark cycle (7:00–19:00 h), with food and water ad libitum. Younger mice (4-6 months) are implanted with i.c.v. cannulas for application of Scopolamine (SCOP) and Scopoletin. The aged mice are injected with Scopoletin by the s.c. route. Experiments are conducted between 8:00 and 16:00 h. Mice with i.c.v. cannulas are randomly divided into four experimental groups: vehicle; SCOP 20 μg; Scopoletin 2 μg; and SCOP 20 μg plus Scopoletin 2 μg. The drugs are applied in 1 μL of vehicle solution (SCOP: saline, Scopoletin: 3 DMSO: 7 sterile water). I.c.v. injections are carried out 15 min before the start of the tests. Aged mice obtained Scopoletin s.c. 30 min prior to object memory test (vehicle: 1 DMSO: 1 EtOH, diluted with olive oil as required)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)	[1]. Hornick A, et al. The coumarin Scopoletin potentiates acetylcholine release from synaptosomes, amplifies hippocampal long-term potentiation and ameliorates anticholinergic- and age-impaired memory. Neuroscience. 2011 Dec 1;197:280-92. [Content Brief] [2]. Liu XL, et al. Effect of Scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001 Oct;22(10):929-33. [Content Brief]

Scopoletin 相關(guān)分類

Inflammation/Immunology

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.2037 mL	26.0186 mL	52.0373 mL	130.0931 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL	26.0186 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL	13.0093 mL
	15 mM	0.3469 mL	1.7346 mL	3.4692 mL	8.6729 mL
	20 mM	0.2602 mL	1.3009 mL	2.6019 mL	6.5047 mL
	25 mM	0.2081 mL	1.0407 mL	2.0815 mL	5.2037 mL
	30 mM	0.1735 mL	0.8673 mL	1.7346 mL	4.3364 mL
	40 mM	0.1301 mL	0.6505 mL	1.3009 mL	3.2523 mL
	50 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6019 mL
	60 mM	0.0867 mL	0.4336 mL	0.8673 mL	2.1682 mL
	80 mM	0.0650 mL	0.3252 mL	0.6505 mL	1.6262 mL
	100 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Scopoletin92-61-5Gelseminic acid Chrysatropic acid東莨菪內(nèi)酯莨菪亭東莨菪亭Cholinesterase (ChE)ApoptosisInhibitorinhibitorinhibit

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Scopoletin (Synonyms: 東莨菪內(nèi)酯; Gelseminic acid; Chrysatropic acid)

Customer Review

Other Forms of Scopoletin:

Scopoletin 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

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摩爾計(jì)算器

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