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  2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Vanillin

Vanillin  (Synonyms: 香蘭素; p-Vanillin; m-Methoxy-p-hydroxybenzaldehyde; p-Hydroxy-m-methoxybenzaldehyde)

目錄號: HY-N0098 純度: 99.97%
COA 產(chǎn)品使用指南

Vanillin (p-Vanillin) 是從香草豆中提取的單個分子，也是在香水，食品和活性分子中廣泛使用的氣味。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Vanillin Chemical Structure

Vanillin Chemical Structure

CAS No. : 121-33-5

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     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥165
In-stock
5 g ￥150
In-stock
10 g ￥240
In-stock
50 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Vanillin:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Vanillin (p-Vanillin) is a single molecule extracted from vanilla beans and also a popular odor used widely in perfume, food and medicine.

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
16.42 μg/mL
Compound: Vanillin
Cytotoxicity against human A549 cells after 3 days by MTT assay
Cytotoxicity against human A549 cells after 3 days by MTT assay
[PMID: 19691312]
B16-4A5 IC50
49.2 μM
Compound: 4-Hydroxy-3-methoxybenzaldehyde
Antimelanogenic activity in theophylline-stimulated mouse B16-4A5 cells assessed as inhibition of melanogenesis after 72 hrs relative to control
Antimelanogenic activity in theophylline-stimulated mouse B16-4A5 cells assessed as inhibition of melanogenesis after 72 hrs relative to control
[PMID: 27756508]
Ca9-22 IC50
> 20 μg/mL
Compound: Vanillin
Cytotoxicity against human Ca9-22 cells after 3 days by MTT assay
Cytotoxicity against human Ca9-22 cells after 3 days by MTT assay
[PMID: 19691312]
Hep 3B2 IC50
> 20 μg/mL
Compound: Vanillin
Cytotoxicity against human Hep3B cells after 3 days by MTT assay
Cytotoxicity against human Hep3B cells after 3 days by MTT assay
[PMID: 19691312]
HepG2 IC50
> 20 μg/mL
Compound: Vanillin
Cytotoxicity against human HepG2 cells after 3 days by MTT assay
Cytotoxicity against human HepG2 cells after 3 days by MTT assay
[PMID: 19691312]
MCF7 IC50
> 50 μM
Compound: 19
Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
MCF7 IC50
15.69 μg/mL
Compound: Vanillin
Cytotoxicity against human MCF7 cells after 3 days by MTT assay
Cytotoxicity against human MCF7 cells after 3 days by MTT assay
[PMID: 19691312]
MDA-MB-231 IC50
> 20 μg/mL
Compound: Vanillin
Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay
Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay
[PMID: 19691312]
NCI-H460 IC50
> 50 μM
Compound: 19
Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
RAW264.7 IC50
> 50 μM
Compound: 32
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
[PMID: 33939429]
SF-268 IC50
> 50 μM
Compound: 19
Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
體外研究
(In Vitro)

Vanillin 以劑量依賴的方式恢復(fù) UVA 誘導(dǎo)的增殖減少。Vanillin 在測試濃度下沒有凋亡作用。此外，由 UVA 照射引起的 OCT4、NANOG 和 SOX2 表達水平降低均通過 Vanillin 處理得到提高，表明 Vanillin 減弱了 UVA 照射對 hAMSCs 的影響?；跓晒馑孛笀蟾婊驕y定，Vanillin 增加了由 UVA 照射引起的 HRE-熒光素酶報告基因活性降低。此外，用 Vanillin 處理可減弱由 UVA 照射引起的 HIF-1α 表達降低。結(jié)果表明，與受 UVA 照射的對照相比，用 Vanillin 處理 hAMSCs 會顯著減少 PGE2 和 cAMP 的產(chǎn)生[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

抗抑郁處理 4 周后，應(yīng)激+香草醛組和應(yīng)激+氟西汀組不動時間顯著減少 [F (4，42) =34.73，P<0.01；F (4，42) =13.55，P<0.01，分別]。用 Vanillin 或氟西汀處理可顯著緩解 CUMS 模型動物的蔗糖消耗減少[ F (4，42) =12.32，P<0.01；F (4，42) =5.65，P<0.01，分別]。CUMS模型大鼠，應(yīng)激+ Vanillin 組和應(yīng)激+氟西汀組5-HT水平較應(yīng)激組顯著升高[F (4，42) =4.846，P=0.030；F (4，42) =4.846，P=0.036]，而兩組去甲腎上腺素 (NE) 升高但不顯著[F (4，42) =6.977，ns]。與應(yīng)激組相比，應(yīng)激+ Vanillin 組的多巴胺 (DA) 也顯著增加[F (4.42) =6.174，P=0.041][2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

152.15

Formula

C8H8O3
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

香蘭素；香草醛
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 100 mg/mL (657.25 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

H2O 中的溶解度 : 25 mg/mL (164.31 mM; 超聲助溶 (<60°C))

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 6.5725 mL 32.8623 mL 65.7246 mL
5 mM 1.3145 mL 6.5725 mL 13.1449 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.43 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.43 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: 99.97%

參考文獻
Kinase Assay
[1]

hAMSCs are irradiated with the indicated doses of UVA and then incubated with the indicated concentrations of Vanillin for three days under serum-free conditions. After the incubation, the concentrations of PGE2 or cAMP in the culture supernatant are measured using ELISA kits. Culture supernatants are added into 96 well plates. Alkaline phosphatase conjugated PGE2 or cAMP and antibodies to PGE2 or cAMP are added to sample wells and incubated at room temperature for 2 h. The sample wells are then washed with PBS and p-nitrophenyl phosphate (pNpp) substrate solution is added. Finally, the samples are incubated at room temperature for 1 h and their absorbance values are read according to the manufacturer's instructions[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[1]

hAMSCs are irradiated with the indicated doses of UVA and then incubated with 1 to 100 μM of Vanillin for three days under serum-free conditions (in DMEM devoid of serum, at 37°C with 5% CO2). After three days, cell proliferation is measured using BrdU incorporation assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Male Sprague-Dawley rats (200 to 250 g) are used in this study. The animals are divided into three groups with 8 to 10 rats per group: the stress+fluoxetine group; the stress+Vanillin aromatherapy group and the stress (untreated) group. For the stress+fluoxetine group, the animals are administered a daily oral dose (10 mg/kg/d, diluted in distilled water) of the SSRI fluoxetine each morning. For the stress+Vanillin group and the bulbectomy+Vanillin group, Vanillin is administrated in a Plexiglas cylinder 50 cm tall and 35 cm diameter with two layers separated by a porous Plexiglas board. The rat still in its cage is gently placed on the upper layer, and 5 mL of 600 mg/L Vanillin (in distilled water) sprayed on to the floor of the lower layer. Rats in the stress and the control groups receive similar handling to the stress+Vanillin group, but without any odor administrated[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.5725 mL 32.8623 mL 65.7246 mL 164.3115 mL
5 mM 1.3145 mL 6.5725 mL 13.1449 mL 32.8623 mL
10 mM 0.6572 mL 3.2862 mL 6.5725 mL 16.4312 mL
15 mM 0.4382 mL 2.1908 mL 4.3816 mL 10.9541 mL
20 mM 0.3286 mL 1.6431 mL 3.2862 mL 8.2156 mL
25 mM 0.2629 mL 1.3145 mL 2.6290 mL 6.5725 mL
30 mM 0.2191 mL 1.0954 mL 2.1908 mL 5.4771 mL
40 mM 0.1643 mL 0.8216 mL 1.6431 mL 4.1078 mL
50 mM 0.1314 mL 0.6572 mL 1.3145 mL 3.2862 mL
60 mM 0.1095 mL 0.5477 mL 1.0954 mL 2.7385 mL
80 mM 0.0822 mL 0.4108 mL 0.8216 mL 2.0539 mL
100 mM 0.0657 mL 0.3286 mL 0.6572 mL 1.6431 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Vanillin
目錄號：
HY-N0098
需求量：