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  2. Metabolic Enzyme/Protease NF-κB
  3. Endogenous Metabolite NF-κB
  4. Sarsasapogenin

Sarsasapogenin  (Synonyms: 菝葜皂苷元; Parigenin; Sarsagenin)

目錄號: HY-N0073 純度: 99.68%
COA 產(chǎn)品使用指南

Sarsasapogenin 是從中草藥知母中分離到的甾體皂苷元，具有抗糖尿病、抗氧化、抗癌、抗炎等多種功效。

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Sarsasapogenin Chemical Structure

Sarsasapogenin Chemical Structure

CAS No. : 126-19-2

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規(guī)格 價格 是否有貨 數(shù)量
5 mg ￥260
In-stock
10 mg ￥395
In-stock
25 mg ￥750
In-stock
50 mg ￥1200
In-stock
100 mg ￥2100
In-stock
200 mg   詢價  
500 mg   詢價  

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Customer Review

Other Forms of Sarsasapogenin:

查看 NF-κB 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities.

IC50 & Target[3]

NF-κB

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
38.15 μM
Compound: Sarsasapogenin
Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
Bcap37 IC50
> 100 μM
Compound: Sarsasapogenin
Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
Hep 3B2 IC50
> 100 μM
Compound: Sarsasapogenin
Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
HepG2 IC50
40.34 μM
Compound: Sarsasapogenin
Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
MCF7 IC50
49.23 μM
Compound: Sarsasapogenin
Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
THP-1 IC50
3.3 μM
Compound: 3
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
[PMID: 20561785]
U-251 IC50
> 100 μM
Compound: Sarsasapogenin
Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay
[PMID: 28038327]
體外研究
(In Vitro)

Sarsasapogenin (20-80 μM) induces apoptosis via caspase-dependent mitochondrial pathway in heLa cells. Sarsasapogenin also induces apoptosis via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin (60 μM)-induced ROS generation triggers ER stress and mitochondrial dysfunction[1]. Sarsasapogenin potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. Moreover, Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, and polarization of M2 to M1 macrophages[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Sarsasapogenin (20 and 40 mg/kg) significantly restores the sucrose preference deficit induced by olfactory bulbectomy (OB), and increases locomotor activity. Sarsasapogenin groups (20 and 40 mg/kg) have significantly lower immobility times, higher AChE protein expression levels than the OB group. Furthermore, Sarsasapogenin (20 and 40 mg/kg) groups have significantly higher α7-nAChR protein expression, and increases higher α4-nAChR protein expression levels compared to rats in the OB group[2]. Sarsasapogenin (5 or 10 mg/kg, p.o.) inhibits TNBS-induced colon shortening and myeloperoxidase activity in mice, reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

416.64

Formula

C27H44O3
CAS 號
性狀

固體

顏色

White to off-white

結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 3.57 mg/mL (8.57 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

Ethanol 中的溶解度 : 1 mg/mL (2.40 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.4002 mL 12.0008 mL 24.0015 mL
5 mM 0.4800 mL 2.4002 mL 4.8003 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： 0.5% CMC-Na/saline water

    Solubility: 5 mg/mL (12.00 mM); 懸濁液; 超聲助溶

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 99.68%

參考文獻(xiàn)
Cell Assay
[1]

The effect of Sarsasapogenin on cell viability is measured by the MTT assay method. After exposure to Sarsasapogenin in the absence or presence of Z-VAD-FMK pretreatment for the indicated time, cells in 96-well plates are incubated with MTT. The formazan precipitate is dissolved in 200 μL of dimethyl sulfoxide, and the absorbance at 570 nm is measured using a Benchmark microplate reader.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

The mice are randomly divided into 7 groups: normal control, TNBS-induced colitic control groups treated with vehicle, timosaponin AIII (5 or 10 mg/kg), Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg). Each group consists of 6 mice. The colitis is induced by the intrarectal injection of 2.5% (w/v) TNBS solution (100 μL, dissolved in 50% ethanol) into the colon of anesthetized mice using a thin round-tip needle equipped with a syringe. The normal group is treated with vehicle alone instead of TNBS and test agents. If the injected TNBS solution is excreted, the mouse is excluded from the experiment. Timosaponin AIII or Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg) dissolved in 2% tween 80 are orally administered once a day for 3 days after treatment with TNBS. The mice are killed 18 h after the final administration of test agents. The colon is quickly removed, opened longitudinally, and gently washed by PBS. Macroscopic evaluation of the colitis grade is scored (0, no ulcer and no inflammation; 1, no ulceration and local hyperemia; 2, ulceration with hyperemia; 3, ulceration and inflammation at one site only; 4, two or more sites of ulceration and inflammation; 5, ulceration extending more than 2 cm). The colons are stored at ?80°C until used in the experiment for ELISA and immunoblotting.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 2.4002 mL 12.0008 mL 24.0015 mL 60.0038 mL
DMSO 5 mM 0.4800 mL 2.4002 mL 4.8003 mL 12.0008 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Sarsasapogenin
目錄號：
HY-N0073
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