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  2. Apoptosis NF-κB
  3. Apoptosis NF-κB
  4. Astragalin

Astragalin  (Synonyms: 紫云英苷; Astragaline; 3-Glucosylkaempferol; Kaempferol 3-β-D-glucopyranoside)

目錄號(hào): HY-N0015 純度: 99.94%
COA 產(chǎn)品使用指南

Astragalin (Astragaline) 是一種具有抗炎、抗氧化、抗癌、抑菌活性的黃酮類化合物。Astragalin 抑制癌細(xì)胞增殖和遷移，誘導(dǎo)細(xì)胞凋亡 (apoptosis)。Astragalin 具有口服活性，具有保護(hù)神經(jīng)和心臟，抵抗骨質(zhì)疏松的作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Astragalin Chemical Structure

Astragalin Chemical Structure

CAS No. : 480-10-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥1045
In-stock
1 mg ￥361
In-stock
5 mg ￥950
In-stock
10 mg ￥1700
In-stock
50 mg 現(xiàn)貨 詢價(jià)
100 mg   詢價(jià)  
200 mg   詢價(jià)  

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Customer Review

Other Forms of Astragalin:

查看 NF-κB 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Astragalin (Astragaline) a flavonoid with anti-inflammatory, antioxidant, anticancer, bacteriostatic activity. Astragalin inhibits cancer cells proliferation and migration, induces apoptosis. Astragalin is orally active and provides nerve and heart protection, and resistance against and osteoporosis[1].

IC50 & Target

p65

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 10 μM
Compound: 6
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
Cytotoxicity against human A549 cells after 48 hrs by SRB assay
[PMID: 28165740]
BT-549 IC50
> 10 μM
Compound: 6
Cytotoxicity against human BT549 cells after 48 hrs by SRB assay
Cytotoxicity against human BT549 cells after 48 hrs by SRB assay
[PMID: 28165740]
BV-2 IC50
23.5 μM
Compound: 6
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
[PMID: 28165740]
BV-2 IC50
77.36 μM
Compound: 13
Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
[PMID: 23149227]
HCT-15 IC50
23.5 μM
Compound: 11
Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human HCT15 cells after 72 hrs by sulforhodamine B assay
[PMID: 25981689]
Jurkat IC50
> 100 μg/mL
Compound: 9
Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay
Cytotoxicity against human Jurkat T cells after 36 hrs by MTT assay
[PMID: 21546250]
MDCK CC50
> 300 μM
Compound: 8
Cytotoxicity against MDCK cells after 48 hrs by MTT assay
Cytotoxicity against MDCK cells after 48 hrs by MTT assay
[PMID: 19729316]
MDCK EC50
72.8 μM
Compound: 8
Inhibition of Influenza A virus A/PR/8/34 (H1N1) neuraminidase activity measured in infected MDCK cells using MUNANA as substrate assessed as fluorescence intensity after 48 hrs
Inhibition of Influenza A virus A/PR/8/34 (H1N1) neuraminidase activity measured in infected MDCK cells using MUNANA as substrate assessed as fluorescence intensity after 48 hrs
[PMID: 25927664]
RAW264.7 IC50
37.1 μM
Compound: 21
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by griess reaction analysis
[PMID: 24679441]
RAW264.7 IC50
40.6 μM
Compound: 21
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs by sandwich ELISA
[PMID: 24679441]
RAW264.7 IC50
64 μM
Compound: 9
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
[PMID: 21353543]
RAW264.7 IC50
67.1 μM
Compound: 9
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha secretion after 18 hrs
[PMID: 21353543]
SK-MEL-2 IC50
> 10 μM
Compound: 6
Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells after 48 hrs by SRB assay
[PMID: 28165740]
SK-OV-3 IC50
> 10 μM
Compound: 6
Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
[PMID: 28165740]
SW-620 IC50
4.6 μM
Compound: 11
Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human SW620 cells after 72 hrs by sulforhodamine B assay
[PMID: 25981689]
U-251 IC50
37.6 μM
Compound: 11
Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human U251 cells after 72 hrs by sulforhodamine B assay
[PMID: 25981689]
U-87MG ATCC IC50
3.12 μM
Compound: 11
Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay
[PMID: 25981689]
Vero IC50
174.3 μM
Compound: 1
Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis
Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis
[PMID: 21466157]
體外研究
(In Vitro)

Astragalin (0-80 μg/mL; 24 h, 48 h, 72 h) inhibits cancer cells viability and migration at 20, 40, 80 μM, and shows no cytotoxicity to normal human colon epithelial cell lines NCM460[1].
Astragalin (80 μg/mL; 4 h, 8 h) interrupts the NF-κB signaling pathway and inhibits NF-κB P65 transcriptional activity in HCT116 cells stimulated by TNF-α[1].
Astragalin (20, 40, 80 μg/mL; 48 h) induces HCT116 cells apoptosis and arrests cell cycle at G0/G1 phase[1].
Astragalin (20, 40, 80 μg/mL; 48 h) increases the level of apoptin in HCT116 and decreases the level of anti-apoptotic proteins in a dose-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Human colon cancer cell lines (HCT116, LoVo, SW620, SW480, Caco2) and human colon epithelial cell lines NCM460
Concentration: 0, 5, 10, 20, 40, 80 μg/mL
Incubation Time: 24, 48, 72 hours
Result: Inhibits cancer cells (LoVo, SW620, SW480, Caco2, HCT116) activity at 20, 40, 80 μM.
Inhibited HCT116 cells with IC50s of 121.845, 87.908, 18.883 μg/mL at 24, 48, 72 hours, respectively.
Showed no cytotoxicity with NCM460 cells.

Cell Cycle Analysis[1]

Cell Line: Human colon cancer cell HCT116
Concentration: 0, 20, 40, 80 μg/mL
Incubation Time: 48 hours
Result: Arrested cell cycle at G0/G1.

Western Blot Analysis[1]

Cell Line: Human colon cancer cell HCT116
Concentration: 80 μg/mL; pre-treated with 20 ng/mL TNF-α
Incubation Time: 4 or 8 hours
Result: Increased the expression of pro-apoptotic proteins (caspase 3, caspase 6, caspase 7, caspase 8, caspase 9, P53, and Bax) and decreased the expression of anti-apoptotic proteins (cleaved caspase-3 and Bcl-2) in a dose-dependent manner.

Cell Migration Assay [1]

Cell Line: Human colon cancer cell HCT116
Concentration: 0, 20, 40, 80 μg/mL
Incubation Time: 24, 48, 72 hours
Result: Reduced release of MMP-2 and MMP-9 enzymes.
Inhibited cell migration with mobilities of 14.38, 13.44, and 5.29% with 20, 40, and 80?μg/mL concentrations, respectively.
體內(nèi)研究
(In Vivo)

Astragalin (25, 50, and 75 mg/kg; p.o.; once every two days; 25 d) reduces the tumor volumes, tumor weight and tumor formation rates and decreases expression of p-NFκB and p-iκκα in nude mouse model?with human colon cancer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c nude mice (4-week-old) with HCT116 cells (s.c.)[1]
Dosage: 0 mg/kg, 25 mg/kg, 50 mg/kg, 75 mg/kg
Administration: Oral gavage; once every two days; for 25 days
Result: Resulted tumor inhibition rates of 17.43%, 34.89% (p?< 0.01), and 67.06% with 25 mg/kg, 50 mg/kg, 75 mg/kg dose administration.
Showed no effect on mouse weight.
分子量

448.38

Formula

C21H20O11
CAS 號(hào)
性狀

固體

顏色

Light yellow to yellow

中文名稱

紫云英苷；紫云英甙；黃芪苷；山奈酚-3-葡萄糖苷
結(jié)構(gòu)分類
初始來(lái)源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 100 mg/mL (223.03 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.58 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.58 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請(qǐng)依序添加每種溶劑： 1% CMC-Na/saline water

    Solubility: 10 mg/mL (22.30 mM); 懸濁液; 超聲助溶

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.94%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2303 mL 11.1513 mL 22.3025 mL 55.7563 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL 11.1513 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL 5.5756 mL
15 mM 0.1487 mL 0.7434 mL 1.4868 mL 3.7171 mL
20 mM 0.1115 mL 0.5576 mL 1.1151 mL 2.7878 mL
25 mM 0.0892 mL 0.4461 mL 0.8921 mL 2.2303 mL
30 mM 0.0743 mL 0.3717 mL 0.7434 mL 1.8585 mL
40 mM 0.0558 mL 0.2788 mL 0.5576 mL 1.3939 mL
50 mM 0.0446 mL 0.2230 mL 0.4461 mL 1.1151 mL
60 mM 0.0372 mL 0.1859 mL 0.3717 mL 0.9293 mL
80 mM 0.0279 mL 0.1394 mL 0.2788 mL 0.6970 mL
100 mM 0.0223 mL 0.1115 mL 0.2230 mL 0.5576 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Astragalin
目錄號(hào)：
HY-N0015
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