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  2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Neuronal Signaling Membrane Transporter/Ion Channel
  3. Glucocorticoid Receptor Endogenous Metabolite iGluR
  4. Corticosterone

Corticosterone  (Synonyms: 皮質(zhì)酮; 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)

目錄號(hào): HY-B1618 純度: 99.76%
COA 產(chǎn)品使用指南 技術(shù)支持

Corticosterone (17-Deoxycortisol) 是一種具有口服活性的，由腎上腺皮質(zhì)產(chǎn)生的糖皮質(zhì)激素，在調(diào)節(jié)邊緣系統(tǒng) (包括海馬體、前額葉皮層和杏仁核) 的神經(jīng)元功能方面起著重要作用。皮質(zhì)酮可通過 SGK 誘導(dǎo)的 GDI 磷酸化增加 Rab 介導(dǎo)的 AMPAR 膜運(yùn)輸。Corticosterone 還能干擾樹突狀細(xì)胞的成熟，具有較好的免疫抑制活性。

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Corticosterone Chemical Structure

Corticosterone Chemical Structure

CAS No. : 50-22-6

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10 mM * 1 mL in DMSO ￥730
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50 mg ￥660
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100 mg ￥1000
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Customer Review

Other Forms of Corticosterone:

MCE 顧客使用本產(chǎn)品發(fā)表的 35 篇科研文獻(xiàn)

WB

    Corticosterone purchased from MCE. Usage Cited in: Sci Adv. 2022 Nov 11;8(45):eadd7063.  [Abstract]

    Corticosterone (CORT) (10 μM, 24 h) is used to produce a cell stress model on the primary culture hippocampal neurons from rats. The decreased NHE1 expression levels in primary cell culture induced by CORT treatment are not affected by chloroquine (Fig. D) but significantly reversed with MG132 pretreatment (Fig. E).

    查看 iGluR 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect[1][2][3][4].

    IC50 & Target

    Human Endogenous Metabolite

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    HEK293 IC50
    1.6 μM
    Compound: corticosterone
    Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells
    Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells
    		[PMID: 35163125]
    體外研究
    (In Vitro)

    Corticosterone (100 nM; 30 min) via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane[1].
    Corticosterone (CORT) (1 μM; 48 h) shows good immunosuppressive properties (functionally compromises maturation of BMDC), which impairs LPS-induced up-regulation of maturation-associated markers (MHC class II, B7.2, B7.1 and CD40)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: HEK293 cells
    Concentration: 100 nM
    Incubation Time: 30 min
    Result: Caused a significant enhancement of mEPSC amplitude (mEPSC represents the postsynaptic response to release of individual vesicles of glutamate).
    Increased the transmission of glutamatergic, and increased synaptic AMPAR currents via a Rab4-dependent mechanism.
    Profoundly increased surface GluR1 cluster density, cluster size and cluster fluorescence intensity.
    Significantly increased the amount of Rab4 that binded to WT-GDI, S45A-GDI, or S121A-GDI but not S213A-GDI.
    Induced the phosphorylation of GST-WTGDI, GST-S45AGDI, and GST-S121AGDI, but not GST-S213AGDI, and this effect was blocked in cells transfected with SGK1 small interfering RNA.
    Increased AMPAR surface expression via a mechanism dependent on GDI phosphorylation.

    Cell Viability Assay[2]

    Cell Line: BMDC cells
    Concentration: 1 μM
    Incubation Time: 48 h
    Result: Completely blocked the expression of MHC class II and B7.2 that induced by LPS, and maximally impaired BMDC cells maturation at 12 h.
    Reduced B7.1 by 50%, and slightly down-regulated CD40.
    體內(nèi)研究
    (In Vivo)

    Corticosterone results in a marked reduction in the ability of BMDC cells to prime naive CD8+ T cells in vivo[2].
    Corticosterone (0.03 or 1 mg/kg; s.c.; single) downregulates expression of BDNF mRNA in dentate gyrus and CA1 of rats[3].
    Corticosterone (2.6 mg/kg; in animal feedings; 8 days) restores ethanol intake and preference to approximately normal preoperative levels in adrenalectomy (ADX) rats[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Wistar rats (150-170 g; adrenalectomized)[3].
    Dosage: 0.03 or 1 mg/kg
    Administration: Subcutaneous injection; single.
    Result: Decreased expression of BDNF mRNA in dentate gyrus, with 25% and 50% lower for dosages of 0.03 and 1 mg/kg, respectively (3 h after administration).
    Reduced approximately 40% BDNF mRNA level as compared to the t=0 h control group (3 h after administration), but the level increased by 100% when 12 h after administration (compared to t=3 h and t=6 h group).
    Animal Model: Male Wistar rats (3-week-old; adrenalectomized)[4].
    Dosage: 2.6 mg/kg
    Administration: In animal feedings; 8 days.
    Result: Restored ethanol intake and preference of adrenalectomy (ADX) rats to approximately normal preoperative levels and to the levels observed in the sham-operated group (SH) rats.
    Clinical Trial
    分子量

    346.46

    Formula

    C21H30O4
    CAS 號(hào)
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    皮質(zhì)酮；皮質(zhì)甾酮；皮質(zhì)脂酮
    結(jié)構(gòu)分類
    初始來源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 100 mg/mL (288.63 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

    Ethanol 中的溶解度 : 14.29 mg/mL (41.25 mM; 超聲助溶)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.8863 mL 14.4317 mL 28.8634 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.22 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.22 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： 0.5% CMC-Na/saline water

      Solubility: 2 mg/mL (5.77 mM); 懸濁液; 超聲助溶 (<60°C)

    • 方案 二

      請(qǐng)依序添加每種溶劑： 20% HP-β-CD in Saline

      Solubility: 4 mg/mL (11.55 mM); 懸濁液; 超聲助溶

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
    請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
    請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.76%

    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.8863 mL 14.4317 mL 28.8634 mL 72.1584 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL 14.4317 mL
    10 mM 0.2886 mL 1.4432 mL 2.8863 mL 7.2158 mL
    15 mM 0.1924 mL 0.9621 mL 1.9242 mL 4.8106 mL
    20 mM 0.1443 mL 0.7216 mL 1.4432 mL 3.6079 mL
    25 mM 0.1155 mL 0.5773 mL 1.1545 mL 2.8863 mL
    30 mM 0.0962 mL 0.4811 mL 0.9621 mL 2.4053 mL
    40 mM 0.0722 mL 0.3608 mL 0.7216 mL 1.8040 mL
    DMSO 50 mM 0.0577 mL 0.2886 mL 0.5773 mL 1.4432 mL
    60 mM 0.0481 mL 0.2405 mL 0.4811 mL 1.2026 mL
    80 mM 0.0361 mL 0.1804 mL 0.3608 mL 0.9020 mL
    100 mM 0.0289 mL 0.1443 mL 0.2886 mL 0.7216 mL
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    產(chǎn)品名稱：
    Corticosterone
    目錄號(hào)：
    HY-B1618
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