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  2. Cell Cycle/DNA Damage Anti-infection Autophagy Apoptosis
  3. Topoisomerase Bacterial Autophagy Apoptosis
  4. Etoposide phosphate

Etoposide phosphate  (Synonyms: 磷酸依托泊苷; BMY-40481)

目錄號: HY-13630 純度: 96.16%
COA 產(chǎn)品使用指南

Etoposide phosphate (BMY-40481) 是一種有效的抗癌 (anti-cancer) 化療試劑和一種選擇性拓?fù)洚悩?gòu)酶 II (topoisomerase II) 抑制劑，可以防止 DNA 鏈的重新連接。Etoposide phosphate 是依托泊苷的磷酸酯前體，被認(rèn)為與 Etoposide 活性相當(dāng)。Etoposide phosphate 誘導(dǎo)細(xì)胞周期阻滯、凋亡 (apoptosis) 和自噬 (autophagy)。

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Etoposide phosphate Chemical Structure

Etoposide phosphate Chemical Structure

CAS No. : 117091-64-2

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in Water ￥1177
In-stock
5 mg ￥800
In-stock
10 mg ￥1300
In-stock
25 mg ￥2600
In-stock
50 mg ￥4100
In-stock
100 mg 現(xiàn)貨 詢價
200 mg   詢價  
500 mg   詢價  

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Customer Review

Other Forms of Etoposide phosphate:

MCE 顧客使用本產(chǎn)品發(fā)表的 102 篇科研文獻(xiàn)

WB
RT-PCR
IF
Proliferation Assay
Cell Viability Assay

    Etoposide phosphate purchased from MCE. Usage Cited in: Oncogene. 2023 Feb 2.  [Abstract]

    Etoposide (VP16; 5?mg/kg; s.c.; every two days for 8 days) strongly suppresses tumor growth in mice.

    Etoposide phosphate purchased from MCE. Usage Cited in: J Pharm Pharmacol. 2023 Mar 1;rgad007.  [Abstract]

    Irinotecan (CPT11; 20 μM; 48 h) or Etoposide (VP16; 20 μM; 48 h) induces MDA-MB-231 cells cycle arrest significantly.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Etoposide induced BCL6 protein expression in a time-dependent manner. ETO-resistant or -sensitive cells are treated with etoposide at their respective 1/4 IC50s for 2, 4, or 6 days.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    STAT1 protein and its phosphorylation levels by immunoblotting analysis. Etoposide (ETO)-resistant and -sensitive cells are treated with Etoposide at their respective 1/2 IC50s for indicated time points.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Etoposide activated mTOR signaling components in Etoposide-resistant Capan-2 and PANC28 cells. Cells were treated with etoposide at their respective 1/2 IC50s for 6 or 12 hr. A long-term treatment with Etoposide activated mTOR signaling components in ETO-resistant cells. Capan-2 and PANC28 cells are treated with 10 μM Etoposide for 2 or 4 days.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    BCL6 mRNA expression in Etoposide (ETO)-resistant and -sensitive cells. Cells are treated with Etoposide at their respective 1/2 IC50s for 24 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Elife. 2022 May 3;11:e69255.  [Abstract]

    Cell sensitivity to Etoposide (ETO). Cancer cells are treated with etoposide at gradient concentrations for 48 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Protein Cell. 2022 Jan;13(1):47-64.  [Abstract]

    FISH of telomeres on metaphase spreads to detect chromosome end-to-end fusions in 6 kb-HeLa treated with VP-16. Control (Scr) or cGAS-depleted cells (gcGAS-1, gcGAS-2) are either harvested immediately following treatment (VP-16, 2 μM, 1 h) or released for 4 h (Release 4 h) during mitosis.

    Etoposide phosphate purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Mar 12;12(3):265.  [Abstract]

    Inhibition of PRDX1 increased the sensitivity of HCT116 cells to Etoposide-induced apoptosis: HCT116 cells infected with lentivirus expressing the indicated shRNAs were treated with 50?μM Etoposide for 0-6?h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Sep 28;7(21):2001364.  [Abstract]

    Lnc‐Ip53 attenuated the DNA damage- and oxidation stress-induced p53 acetylation at K382. Cells are incubated with 50 μM Etoposide (Eto) for 5 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Sep 28;7(21):2001364.  [Abstract]

    Lnc‐Ip53 attenuated the DNA damage‐ and oxidation stress‐triggered increase of CDKN1A and PUMA. Cells are incubated with 50 μM Etoposide (Eto) for 6 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Hepatology. 2020 May;71(5):1660-1677.  [Abstract]

    Exposure to Etoposide (Eto), another DNA-damaging agent, induces PDIA3P1 expression, suggesting that PDIA3P1 may be up-regulated by DNA-damaging agents.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    When cells are treated with 20 μM Etoposide, an obvious increased green focus of RAD51 is observed in the treated fibroblasts using immunofluorescence (IF) staining.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    Fibroblasts and HT-1080 cells are treated with 20 μM Etoposide or DMSO (vehicle group) for 2, 4, 8, 16, 24 and 36 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Theranostics. 2020 Jul 25;10(21):9477-9494.  [Abstract]

    When cells are treated with 20 μM Etoposide or DMSO (vehicle group) for 4 h, western blots (WB) shows elevated expression of γH2AX, a marker of double-strand breaks (DSBs), in the Etoposide group.

    Etoposide phosphate purchased from MCE. Usage Cited in: EMBO Mol Med. 2020 Nov 6;12(11):e12525.  [Abstract]

    Immunoblot of PARP1 cleavage of PeTa cells after 6 days of indicated treatment. Etoposide (10 μM; for 24 h) serves as positive control for apoptosis, and H3 serves as loading control.

    Etoposide phosphate purchased from MCE. Usage Cited in: Aging Cell. 2020 Jul;19(7):e13171.  [Abstract]

    RPE p16‐mCherry cells were treated with DMSO, Etoposide (20 μM), H2O2 (200 μM), or serum starved for 24 hr.

    Etoposide phosphate purchased from MCE. Usage Cited in: Leuk Lymphoma. 2018 Jan;59(1):162-170.  [Abstract]

    WT1 and caspase-3 protein levels are detected in two primary AML blasts treated with 100 μM Eto for 24 h.

    Etoposide phosphate purchased from MCE. Usage Cited in: Cancer Lett. 2017 Nov 1;408:43-54.  [Abstract]

    Western blot analysis of p-p70S6k, p70S6k, p-AKT and AKT after 6 h of treatment with 20 μM RAD001. Levels of p-p70S6k and p-AKT are quantified by densitometric analysis and a corresponding histogram is constructed as relative to p70S6k or AKT and α-tubulin. The lower panel shows a representative Western blot.

    查看 Topoisomerase 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Etoposide phosphate (BMY-40481) is a potent anti-cancer chemotherapy agent and a selective topoisomerase II inhibitor to prevent re-ligation of DNA strands. Etoposide phosphate is the phosphate ester proagent of etoposide and is considered as active equivalent to Etoposide. Etoposide phosphate induces cell cycle arrest, apoptosis, and autophagy.

    IC50 & Target[1]

    Topoisomerase II

     

    體外研究
    (In Vitro)

    Etoposide phosphate 是依托泊苷的水溶性衍生物，可能是依托泊苷的前體藥物，其特征在于在依托泊苷分子 E 環(huán)的 4' 位存在磷酸基團(tuán)[1]。
    Etoposide phosphate (0-1 μM；72 小時) 抑制 HCT116 FBXW+/+、FBXW-/- 和 p53-/- 作為劑量-依賴方式，表現(xiàn)出 IC50 值為 0.945 μM；0.375 微米；和 1.437 μM[2]。
    Etoposide phosphate (25 μM；6 小時) 延遲 FBXW7 缺陷細(xì)胞中的 p53 恢復(fù)。此外，F(xiàn)BXW7-/- 細(xì)胞中 FBXW7 表達(dá)消失[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: FBXW+/+, FBXW-/- and p53-/- cell
    Concentration: 0.025 μM, 0.05 μM, 0.075 μM, 0.1 μM, 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM, 1 μM
    Incubation Time: 72 hours
    Result: Inhibited HCT116 FBXW+/+, FBXW-/- and p53-/- cell growth as a concentration manner.

    Western Blot Analysis[2]

    Cell Line: HCT116 FBXW7+/+ or FBXW7-/- cells
    Concentration: 25 μM
    Incubation Time: 6 hours
    Result: Exhibited that the recovery of p53 levels after DNA damage is mediated by FBXW7.
    體內(nèi)研究
    (In Vivo)

    磷酸依托泊苷 (靜脈注射；50、100 或 150 mg/kg；單劑量) 在第 8 天雌性 CD-1 小鼠中出現(xiàn)進(jìn)行性共濟(jì)失調(diào)、翻正反射受損、前后肢張開和麻痹的臨床癥狀[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female CD-1 mice[3]
    Dosage: 50, 100, or 150 mg/kg
    Administration: Intravenous injection; single dose
    Result: Observed degeneration of dorsal root ganglion cells and axonal degeneration of their distal and proximal processes in peripheral nerves, dorsal spinal roots, and dorsal funiculi of the spinal cord at all doses under light microscopy (LM).
    Clinical Trial
    分子量

    668.54

    Formula

    C29H33O16P
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    磷酸依托泊苷
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (149.58 mM; 超聲助溶 (<80°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : 100 mg/mL (149.58 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.4958 mL 7.4790 mL 14.9580 mL
    5 mM 0.2992 mL 1.4958 mL 2.9916 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 100 mg/mL (149.58 mM); 澄清溶液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料
    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.4958 mL 7.4790 mL 14.9580 mL 37.3949 mL
    5 mM 0.2992 mL 1.4958 mL 2.9916 mL 7.4790 mL
    10 mM 0.1496 mL 0.7479 mL 1.4958 mL 3.7395 mL
    15 mM 0.0997 mL 0.4986 mL 0.9972 mL 2.4930 mL
    20 mM 0.0748 mL 0.3739 mL 0.7479 mL 1.8697 mL
    25 mM 0.0598 mL 0.2992 mL 0.5983 mL 1.4958 mL
    30 mM 0.0499 mL 0.2493 mL 0.4986 mL 1.2465 mL
    40 mM 0.0374 mL 0.1870 mL 0.3739 mL 0.9349 mL
    50 mM 0.0299 mL 0.1496 mL 0.2992 mL 0.7479 mL
    60 mM 0.0249 mL 0.1246 mL 0.2493 mL 0.6232 mL
    80 mM 0.0187 mL 0.0935 mL 0.1870 mL 0.4674 mL
    100 mM 0.0150 mL 0.0748 mL 0.1496 mL 0.3739 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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    產(chǎn)品名稱：
    Etoposide phosphate
    目錄號：
    HY-13630
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