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  1. Anti-infection
  2. Enterovirus
  3. Brilliant Black BN

Brilliant Black BN  (Synonyms: E 151)

目錄號: HY-128382 純度: ≥98.0%
COA 產(chǎn)品使用指南

Brilliant black BN (E151) 是一種偶氮染料 dye 和一種食品著色劑。Brilliant black BN (E151) 是一種很有前途的抗病毒試劑 (antiviral agent ),通過抑制 EV71 與其細(xì)胞脫衣因子親環(huán)素a的相互作用來對抗 EV71 感染。Brilliant black BN (E151) 有潛力用于傳染性疾病的相關(guān)研究。

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Brilliant Black BN Chemical Structure

Brilliant Black BN Chemical Structure

CAS No. : 2519-30-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ¥470
In-stock
5 mg ¥378
In-stock
10 mg ¥492
In-stock
25 mg ¥788
In-stock
50 mg ¥1220
In-stock
100 mg   詢價(jià)  
200 mg   詢價(jià)  

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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Brilliant black BN (E151) is an azo dye and a food colorant[1]. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease[2].

IC50 & Target

IC50: 2.39 μM- 28.12 μM (various strains of EV71)[2]

體外研究
(In Vitro)

Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A [1].
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 μM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC50 value of 10.1 μM[1].
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 μM to 28.12 μM ,whereas its 50% cytotoxic concentration is 1870 μM [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 14-days old AG129 mice with EV71[1]
Dosage: 200 mg/kg
Administration: Intraperitoneal injection
Result: Had a Protective eddect on EV71 challenged AG129 mice by dye E151.
分子量

867.68

Formula

C28H17N5Na4O14S4

CAS 號
性狀

固體

顏色

Brown to black

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn): 

DMSO 中的溶解度 : 125 mg/mL (144.06 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響,請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.1525 mL 5.7625 mL 11.5250 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲存時(shí),請?jiān)?個(gè)月內(nèi)使用,-20°C儲存時(shí),請?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計(jì)算器
請輸入動物實(shí)驗(yàn)的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實(shí)驗(yàn)過程有損耗,建議您多配一只動物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲存時(shí),請?jiān)?個(gè)月內(nèi)使用,-20°C儲存時(shí),請?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1525 mL 5.7625 mL 11.5250 mL 28.8125 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL 5.7625 mL
10 mM 0.1152 mL 0.5762 mL 1.1525 mL 2.8812 mL
15 mM 0.0768 mL 0.3842 mL 0.7683 mL 1.9208 mL
20 mM 0.0576 mL 0.2881 mL 0.5762 mL 1.4406 mL
25 mM 0.0461 mL 0.2305 mL 0.4610 mL 1.1525 mL
30 mM 0.0384 mL 0.1921 mL 0.3842 mL 0.9604 mL
40 mM 0.0288 mL 0.1441 mL 0.2881 mL 0.7203 mL
50 mM 0.0230 mL 0.1152 mL 0.2305 mL 0.5762 mL
60 mM 0.0192 mL 0.0960 mL 0.1921 mL 0.4802 mL
80 mM 0.0144 mL 0.0720 mL 0.1441 mL 0.3602 mL
100 mM 0.0115 mL 0.0576 mL 0.1152 mL 0.2881 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
Brilliant Black BN
目錄號:
HY-128382
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