Inz-1 is a potent and selective mitochondrial cytochrome bc1 inhibitor for yeast (IC₅₀=8.092 μM) over humans (IC₅₀=45.320 μM). Inz-1 reverses Fluconazole (HY-B0101) or other triazole antifungals’ resistance in?the pathogenic fungus Candida albicans^[1].

IC50: 8.092 μM (yeast mitochondrial cytochrome bc1)
IC50: 45.320 μM (human mitochondrial cytochrome bc1)^[1]

Inz-1 (0-16 μM; 24 hours) inhibits the growth of the model yeast?S. cerevisiae?(strain BY4741) and?C. albicans?(SC5314) in media containing glucose or glycerol as the sole carbon source, while only mildly slowing growth in glucose^[1].?
Inz-1 (0-100 μM; 24 hours) shows inhibition of yeast cytochrome B enzymatic activity in a concentration -dependent manner in both wild-type and F90Y mutant mitochondria. It exhibits inhibition of cytochrome bc1 from both?S. cerevisiae and?C. albicans?with IC₅₀ value of 2.5 μM and 8.0 μM, respectively^[1].
Inz-1 (0-100 μM; 24 hours) is selective for yeast over human cytochrome bc1, it inhibits human cytochrome bc1 activity only weakly, with an IC₅₀?of 45.3 μM, 5.6-fold higher than the IC₅₀?for the?C. albicans?homolog in mitochondria purified from HEK293 human embryonic kidney cells^[1].
Inz-1 (0-100 μM; 24 hours) inhibits proliferation under forced respiration conditions only at 32 μM，and only mildly at that concentration^[1].
Inz-1 is against candida, human enzyme with IC₅₀ values of 8.092 μM and 45.320 μM, respectively. It against Candida and HepG2 respiring growth with IC₅₀ values of 1.655 uM and >32 μM, respectively^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

266.29

C₁₆H₁₄N₂O₂

897776-15-7

Room temperature in continental US; may vary elsewhere.

• [1]. Benjamin M Vincent, et al. A Fungal-Selective Cytochrome Bc 1 Inhibitor Impairs Virulence and Prevents the Evolution of Drug Resistance.Cell Chem Biol. 2016 Aug 18;23(8):978-991.  [Content Brief]