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  2. 誘導(dǎo)疾病模型產(chǎn)品 Membrane Transporter/Ion Channel Neuronal Signaling Autophagy Apoptosis Metabolic Enzyme/Protease
  3. 神經(jīng)系統(tǒng)疾病模型 TRP Channel Autophagy Apoptosis Endogenous Metabolite
  4. 神經(jīng)性疼痛模型
  5. Capsaicin

Capsaicin  (Synonyms: 辣椒素; (E)-Capsaicin)

目錄號: HY-10448 純度: 99.85%
COA 產(chǎn)品使用指南

Capsaicin ((E)-Capsaicin) 是辣椒中的活性成分，是一種 TRPV1 激動劑。Capsaicin 具有減輕疼痛、抗氧化、抗炎、抗癌和一定的神經(jīng)毒性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Capsaicin Chemical Structure

Capsaicin Chemical Structure

CAS No. : 404-86-4

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥990
In-stock
50 mg ￥900
In-stock
100 mg ￥1530
In-stock
200 mg ￥2600
In-stock
500 mg   詢價  
1 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Capsaicin:

MCE 顧客使用本產(chǎn)品發(fā)表的 41 篇科研文獻(xiàn)

WB
Cell Viability Assay

    Capsaicin purchased from MCE. Usage Cited in: Theranostics. 2020 Jun 24;10(17):7906-7920.  [Abstract]

    CNE2 and SUNE1 cell growth is inhibited by Capsaicin in a dose-dependent manner.

    Capsaicin purchased from MCE. Usage Cited in: Theranostics. 2020 Jun 24;10(17):7906-7920.  [Abstract]

    The expression of cell cycle-associated molecules is altered by Capsaicin treatment in both cell lines.The cell-cycle inhibitor p27Kip1 is upregulated after Capsaicin treatment while the G1-S phase transition marker cyclin D1 is downregulated. p38 phosphorylation levels are also decreased following capsaicin treatment.

    Capsaicin purchased from MCE. Usage Cited in: Clin Exp Pharmacol Physiol. 2017 Jul;44(7):803-814.  [Abstract]

    Western blot analysis showing that the CAP (0.5 mg/kg) injection inhibits the increase of p65 expression and phosphorylation at 24 h after I/R injury.

    查看 TRP Channel 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Capsaicin ((E)-Capsaicin), an active component of chili peppers, is a TRPV1 agonist. Capsaicin has pain-relieving, antioxidant, anti-inflammatory, anti-cancer and certain neurotoxic effects[1][2].

    IC50 & Target

    EC50: 290 nM (hTRPV1, in HEK293 cell)[1]
    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A2058 IC50
    > 100 μM
    Compound: Capsaicin
    Cytotoxicity against human A2058 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against human A2058 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 31104785]
    B16-F10 IC50
    > 100 μM
    Compound: Capsaicin
    Cytotoxicity against mouse B16F10 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against mouse B16F10 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 31104785]
    BGC-823 IC50
    4.659 μM
    Compound: 1
    Antiproliferative activity against human BGC-823 cells assessed as reduction in cell viability incubated for 5 days by MTS assay
    Antiproliferative activity against human BGC-823 cells assessed as reduction in cell viability incubated for 5 days by MTS assay
    		[PMID: 33581557]
    CHO EC50
    25.9 nM
    Compound: 1; CAP
    Agonist activity at human TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method
    Agonist activity at human TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method
    		[PMID: 31702924]
    CHO EC50
    44.8 nM
    Compound: 1; CAP
    Agonist activity at rat TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method
    Agonist activity at rat TRPV1 expressed in CHO cells assessed as increase in 45Ca2+ uptake incubated for 5 mins by liquid scintillation counting method
    		[PMID: 31702924]
    CHO EC50
    44.8 nM
    Compound: Capsaicin
    In vitro [Ca2+] influx relative to capsaicin by Rat Vanilloid receptor (VR1) expressing CHO cells
    In vitro [Ca2+] influx relative to capsaicin by Rat Vanilloid receptor (VR1) expressing CHO cells
    		[PMID: 12825950]
    HBMEC-2 EC50
    16.37 μM
    Compound: Capsaicin
    Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    Neuroprotective activity against H2O2-induced cell damage in human HBMEC2 cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    		[PMID: 31541868]
    HCT-116 IC50
    40.16 μM
    Compound: Capsaicin
    Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    		[PMID: 34795858]
    HEK293 EC50
    0.0039 μM
    Compound: Capsaicin
    Agonist activity at human TRPV1 ion channel expressed in HEK293 cells assessed as calcium influx by fluo-4-Am-based fluorimetry
    Agonist activity at human TRPV1 ion channel expressed in HEK293 cells assessed as calcium influx by fluo-4-Am-based fluorimetry
    		[PMID: 22257892]
    HEK293 EC50
    0.0053 μM
    Compound: 1a
    Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as Ca2+ influx by Fluo-4 dye based assay
    Agonist activity at human recombinant TRPV1 expressed in HEK293 cells assessed as Ca2+ influx by Fluo-4 dye based assay
    		[PMID: 25666822]
    HEK293 IC50
    0.008 μM
    Compound: 1a
    Antagonist activity against human recombinant TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay
    Antagonist activity against human recombinant TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay
    		[PMID: 25666822]
    HeLa IC50
    > 50 μM
    Compound: CAP
    Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
    Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
    		[PMID: 29685681]
    K562 IC50
    49.41 μM
    Compound: CAP
    Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
    Antiproliferative activity against human K562 cells after 48 hrs by MTT assay
    		[PMID: 29685681]
    MCF7 IC50
    100 μM
    Compound: Capsaicin
    Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition by MTT assay
    Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition by MTT assay
    		[PMID: 33508189]
    MCF7 IC50
    49.97 μM
    Compound: CAP
    Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 29685681]
    MDA-MB-231 IC50
    120 μM
    Compound: 24
    Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay
    Antiproliferative activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay
    		[PMID: 32686940]
    NCI-H460 IC50
    30.66 μM
    Compound: Capsaicin
    Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    		[PMID: 34795858]
    SH-SY5Y EC50
    14.02 μM
    Compound: Capsaicin
    Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    Neuroprotective activity against H2O2-induced cell damage in human SH-SY5Y cells assessed as increase in cell viability preincubated for 24 hrs followed by H2O2 addition and measured after 4 hrs by MTT assay
    		[PMID: 31541868]
    SH-SY5Y EC50
    2.32 μM
    Compound: Capsaicin
    Agonist activity at rat TRPV1 expressed in human SH-SY5Y cells assessed as intracellular calcium accumulation by Fluo-4 NW dye based fluorescence assay
    Agonist activity at rat TRPV1 expressed in human SH-SY5Y cells assessed as intracellular calcium accumulation by Fluo-4 NW dye based fluorescence assay
    		[PMID: 30783490]
    SK-MEL-25 IC50
    > 100 μM
    Compound: Capsaicin
    Cytotoxicity against human SK-MEL-25 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against human SK-MEL-25 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 31104785]
    SK-OV-3 IC50
    22.03 μM
    Compound: Capsaicin
    Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by crystal violet staining based assay
    		[PMID: 34795858]
    T47D IC50
    100 μM
    Compound: Capsaicin
    Antiproliferative activity in human T47D cells assessed as cell growth inhibition by MTT assay
    Antiproliferative activity in human T47D cells assessed as cell growth inhibition by MTT assay
    		[PMID: 33508189]
    U-87MG ATCC IC50
    > 100 μM
    Compound: Capsaicin
    Cytotoxicity against human U87 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against human U87 cells assessed as decrease in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 31104785]
    體外研究
    (In Vitro)

    Capsaicin (50-300 μM；24-72 小時) 表現(xiàn)出劑量和時間依賴性方式細(xì)胞生長的減少。觀察到的 IC50 值約為 150 μM[2]。
    Capsaicin (50-300 μM; 24-72 小時) 顯示細(xì)胞質(zhì)細(xì)胞色素 c 增加、胱天蛋白酶 3 和 PARP (p85) 水平激活，并降低抗凋亡 Bcl-2 蛋白，增加促凋亡 Bad/Bax 表達(dá)[2]。
    Capsaicin 增加核凝聚、核 DNA 碎片和亞 G1 DNA 含量[2]。
    Capsaicin 通過降低細(xì)胞周期蛋白 B1 和 D1 調(diào)節(jié)劑以及細(xì)胞周期蛋白依賴性蛋白激酶 cdk-1、cdk-2 和 cdk-4 的表達(dá)來抑制 FaDu 細(xì)胞在 G1/S 期的細(xì)胞周期進(jìn)程[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: Human pharyngeal squamous carcinoma cells (FaDu) cells
    Concentration: 50 μM, 100 μM, 200 μM, and 300 μM
    Incubation Time: 24 hours, 48 hours and 72 hours
    Result: Showed an augmented decrease in cell growth.

    Apoptosis Analysis[2]

    Cell Line: FaDu cells
    Concentration: 50 μM, 100 μM and 200 μM
    Incubation Time: 12 hours
    Result: Increased the activity of caspase 3 in a time-dependent manner.

    Western Blot Analysis[2]

    Cell Line: FaDu cells
    Concentration: 200 μM
    Incubation Time: 24 hours
    Result: The observed activation of caspase 3 and PARP (p85) levels.
    體內(nèi)研究
    (In Vivo)

    Capsaicin (50-300 μM；24-72 小時) 表現(xiàn)出劑量和時間依賴性方式細(xì)胞生長的減少。觀察到的 IC50 值約為 150 μM[2]。
    Capsaicin (50-300 μM; 24-72 小時) 顯示細(xì)胞質(zhì)細(xì)胞色素 c 增加、胱天蛋白酶 3 和 PARP (p85) 水平激活，并降低抗凋亡 Bcl-2 蛋白，增加促凋亡 Bad/Bax 表達(dá)[2]。
    Capsaicin 增加核凝聚、核 DNA 碎片和亞 G1 DNA 含量[2]。
    Capsaicin 通過降低細(xì)胞周期蛋白 B1 和 D1 調(diào)節(jié)劑以及細(xì)胞周期蛋白依賴性蛋白激酶 cdk-1、cdk-2 和 cdk-4 的表達(dá)來抑制 FaDu 細(xì)胞在 G1/S 期的細(xì)胞周期進(jìn)程[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Swiss albino mice (20-25 g; 8-10 weeks old) induced by Benzo(a)pyrene[3]
    Dosage: 10 mg/kg
    Administration: Intraperitoneal administration; once in a week; for 14 weeks
    Result: Inhibited the development of mice lung carcinogenesis.
    Clinical Trial
    分子量

    305.41

    Formula

    C18H27NO3
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    辣椒素；辣椒堿
    結(jié)構(gòu)分類
    初始來源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 100 mg/mL (327.43 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    Ethanol 中的溶解度 : 100 mg/mL (327.43 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 3.2743 mL 16.3714 mL 32.7429 mL
    5 mM 0.6549 mL 3.2743 mL 6.5486 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實(shí)驗(yàn)：

    請根據(jù)您的 實(shí)驗(yàn)動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% EtOH    90% Corn Oil

      Solubility: ≥ 20 mg/mL (65.49 mM); 澄清溶液

      此方案可獲得 ≥ 20 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個月以上的動物實(shí)驗(yàn)酌情使用。

      以 1 mL 工作液為例，取 100 μL 200.0 mg/mL 的澄清 EtOH 儲備液加到 900 μL 玉米油中，混合均勻。

    • 方案 二

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.19 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    動物溶解方案計算器
    請輸入動物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    您所需的儲備液濃度超過該產(chǎn)品的實(shí)測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.85%

    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 3.2743 mL 16.3714 mL 32.7429 mL 81.8572 mL
    5 mM 0.6549 mL 3.2743 mL 6.5486 mL 16.3714 mL
    10 mM 0.3274 mL 1.6371 mL 3.2743 mL 8.1857 mL
    15 mM 0.2183 mL 1.0914 mL 2.1829 mL 5.4571 mL
    20 mM 0.1637 mL 0.8186 mL 1.6371 mL 4.0929 mL
    25 mM 0.1310 mL 0.6549 mL 1.3097 mL 3.2743 mL
    30 mM 0.1091 mL 0.5457 mL 1.0914 mL 2.7286 mL
    40 mM 0.0819 mL 0.4093 mL 0.8186 mL 2.0464 mL
    50 mM 0.0655 mL 0.3274 mL 0.6549 mL 1.6371 mL
    60 mM 0.0546 mL 0.2729 mL 0.5457 mL 1.3643 mL
    80 mM 0.0409 mL 0.2046 mL 0.4093 mL 1.0232 mL
    100 mM 0.0327 mL 0.1637 mL 0.3274 mL 0.8186 mL
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Capsaicin
    目錄號：
    HY-10448
    需求量：