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  2. GPCR/G Protein
  3. Angiotensin Receptor
  4. L162389

L162389 是一種有效的 angiotensin AT1 受體 拮抗劑，Ki 值為 28 nM。

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L162389 Chemical Structure

L162389 Chemical Structure

CAS No. : 169281-53-2

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查看 Angiotensin Receptor 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

L162389 is a potent antagonist of angiotensin AT1 receptor with Ki of 28 nM.

IC50 & Target

Ki: 28 nM (angiotensin AT1 receptor)[1]
體外研究
(In Vitro)

L-162,389 stimulates phosphatidylinositol turnover, albeit only to a small percentage of the angiotensin response. L-162,389 acts as angiotensin antagonist with IC50 value of 105 nM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

562.72

Formula

C31H38N4O4S
CAS 號
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
純度 & 產(chǎn)品資料
參考文獻
Kinase Assay
[1]

Monoiodinated 125I-[Sar1,Leu8]angiotensin II is prepared by the Iodo-Gen method. One day after transfection and 24 hr before the binding experiments, the transfected cells are transferred to 6-, 12-, or 24-well culture plates, with 0.15-9 × 105 cells/well, with a goal of total binding of 5-10% of the radiolabeled peptide. The cells are washed twice with buffer (25 mM Tris, 5 mM MgCl2, 140 mM NaCl, pH 7.4) before and after the binding. The binding is carried out for 24 hr at 4°C with 50 pm 125I-[Sar1,Leu8]angiotensin II and variable amounts of unlabeled nonpeptide or peptide ligands in 0.5-1 mL of a 25 mM Tris buffer containing 5 mm MgCl2, pH 7.4. The binding data are analyzed by computerized nonlinear regression analysis using InPlot 4.0.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
L162389
目錄號：
HY-101618
需求量：