Spiramycin (Synonyms: 螺旋霉素; Rovamycin)

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Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計(jì)算器

Spiramycin (Rovamycin) 是由鏈霉菌 (Streptomyces ambofaciens) 產(chǎn)生的大環(huán)內(nèi)酯抗生素，具有抗細(xì)菌和抗弓形蟲的作用，并具有抗寄生蟲作用。Spiramycin (Rovamycin) 由 16 個(gè)內(nèi)酯環(huán)組成，其上附著三種糖 (mycaminose，forosamine 和 mycarose)。

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Spiramycin Chemical Structure

CAS No. : 8025-81-8

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Customer Review

Other Forms of Spiramycin:

Spiramycin (hexanedioate) 詢價(jià)
Spiramycin (Standard) 詢價(jià)

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)

•Cell Prolif. 2021 Jan;54(1):e12953. [Abstract]

Spiramycin 相關(guān)產(chǎn)品

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生物活性
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

Spiramycin (Rovamycin) is a macrolide antibiotic produced by Streptomyces ambofaciens with against bacteria and Toxoplasma gondii activities, and also has antiparasitic effect. Spiramycin is composed of a 16-member lactone ring, on which three sugars (mycaminose, forosamine, and mycarose) are attached^[1]^[2].

IC₅₀ & Target

Macrolide

Toxoplasma

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	189 μM Compound: Spiramycin	Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34894691]
HeLa	IC₅₀	34.41 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay	[PMID: 27501415]
HepG2	IC₅₀	30.51 μM Compound: 1	Cytotoxicity against human HepG2 cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay Cytotoxicity against human HepG2 cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay	[PMID: 27501415]
KB	IC₅₀	31.26 μM Compound: 1	Cytotoxicity against human KB cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay Cytotoxicity against human KB cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay	[PMID: 27501415]
MCF7	IC₅₀	33.11 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay	[PMID: 27501415]
NHDF	IC₅₀	31.1 μM Compound: 1	Cytotoxicity against HDF assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay Cytotoxicity against HDF assessed as reduction in cell survival after 72 hrs by sulforhodamine B assay	[PMID: 27501415]

體外研究
(In Vitro)

Spiramycin (24 hours; 1-1000 μM; T. gondii infected HeLa cells and HeLa cells) treatment reduces the cytotoxicity, and shows anti-Toxoplasma gondii activity, with IC₅₀ values of 189 μM for HeLa cells; and 262 μM for T. gondii-infected HeLa cells^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[3]

Cell Line:	T. gondii infected HeLa cells and HeLa cells
Concentration:	1-1000 μM
Incubation Time:	24 hours
Result:	Reduced the cytotoxicity.

體內(nèi)研究
(In Vivo)

Spiramycin (100 mg/kg; intraperitoneal injection; every day; for 4 days; female KM mice) treatment reduces the number of tachyzoites, and reduces hepatotoxicity and significantly enhances antioxidative effects. Spiramycin treatment also decreases in the degree of granulomatous inflammation in the liver^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	36 female KM mice with T.gondii^[3]
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection; every day; for 4 days
Result:	The number of tachyzoites was significantly reduced. Reduced hepatotoxicity and significantly enhanced antioxidative effects. Granuloma and cyst formation were inhibitied.

Clinical Trial

分子量

843.05

Formula

C₄₃H₇₄N₂O₁₄

CAS 號(hào)

8025-81-8

性狀

固體

顏色

White to off-white

中文名稱

螺旋霉素

結(jié)構(gòu)分類

初始來源

微生物

Streptomyces ambofaciens

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

細(xì)胞實(shí)驗(yàn)：

DMSO 中的溶解度 : ≥ 100 mg/mL (118.62 mM; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	1.1862 mL	5.9308 mL	11.8617 mL
5 mM	0.2372 mL	1.1862 mL	2.3723 mL
10 mM	0.1186 mL	0.5931 mL	1.1862 mL

查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

摩爾計(jì)算器
稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.97 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.97 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請(qǐng)依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.97 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個(gè)月以上的動(dòng)物實(shí)驗(yàn)酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL玉米油中，混合均勻。

動(dòng)物溶解方案計(jì)算器

請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只

由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量

請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計(jì)算結(jié)果

工作液所需濃度 : mg/mL

儲(chǔ)備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國技術(shù)支持聯(lián)系。

動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。

請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.19%

選擇批次：

Data Sheet (634 KB) SDS (458 KB)

COA (297 KB) HNMR (336 KB) LCMS (259 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. Nguyen HC, et al. Post-PKS tailoring steps of the spiramycin macrolactone ring in Streptomyces ambofaciens. Antimicrob Agents Chemother. 2013 Aug;57(8):3836-42. [Content Brief]

[2]. Etewa SE, et al. Assessment of spiramycin-loaded chitosan nanoparticles treatment on acute and chronic toxoplasmosis in mice. J Parasit Dis. 2018 Mar;42(1):102-113. [Content Brief]

[3]. Guo HY, et al. Synthesis and Biological Evaluation of (+)-Usnic Acid Derivatives as Potential Anti-Toxoplasma gondii Agents. J Agric Food Chem. 2019 Aug 28;67(34):9630-9642. [Content Brief]

Spiramycin 相關(guān)分類

Infection

完整儲(chǔ)備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1862 mL	5.9308 mL	11.8617 mL	29.6542 mL
	5 mM	0.2372 mL	1.1862 mL	2.3723 mL	5.9308 mL
	10 mM	0.1186 mL	0.5931 mL	1.1862 mL	2.9654 mL
	15 mM	0.0791 mL	0.3954 mL	0.7908 mL	1.9769 mL
	20 mM	0.0593 mL	0.2965 mL	0.5931 mL	1.4827 mL
	25 mM	0.0474 mL	0.2372 mL	0.4745 mL	1.1862 mL
	30 mM	0.0395 mL	0.1977 mL	0.3954 mL	0.9885 mL
	40 mM	0.0297 mL	0.1483 mL	0.2965 mL	0.7414 mL
	50 mM	0.0237 mL	0.1186 mL	0.2372 mL	0.5931 mL
	60 mM	0.0198 mL	0.0988 mL	0.1977 mL	0.4942 mL
	80 mM	0.0148 mL	0.0741 mL	0.1483 mL	0.3707 mL
	100 mM	0.0119 mL	0.0593 mL	0.1186 mL	0.2965 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spiramycin8025-81-8Rovamycin螺旋霉素BacterialParasiteAntibioticInhibitorinhibitorinhibit

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Spiramycin (Synonyms: 螺旋霉素; Rovamycin)

Customer Review

Other Forms of Spiramycin:

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)