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  2. Membrane Transporter/Ion Channel Autophagy Anti-infection Apoptosis
  3. Proton Pump Autophagy Antibiotic Bacterial Apoptosis
  4. Bafilomycin A1

Bafilomycin A1  (Synonyms: 巴佛洛霉素A1; BafA1)

目錄號(hào): HY-100558 純度: 98.67%
COA 產(chǎn)品使用指南

Bafilomycin A1 (BafA1) 是特異性，可逆的 V-ATPase 抑制劑，IC50 值為 4-400 nmol/mg。Bafilomycin A1，大環(huán)內(nèi)酯類抗生素， 是一種自噬 (autophagy) 晚期階段抑制劑。Bafilomycin A1 阻斷自噬體與溶酶體的融合，并抑制培養(yǎng)細(xì)胞溶酶體中的酸化和蛋白質(zhì)降解。Bafilomycin A1 也誘導(dǎo)調(diào)亡 (apoptosis)。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Bafilomycin A1 Chemical Structure

Bafilomycin A1 Chemical Structure

CAS No. : 88899-55-2

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規(guī)格 價(jià)格 是否有貨 數(shù)量
100 μg ￥990
In-stock
500 μg ￥2050
In-stock
1 mg ￥2800
In-stock
5 mg   詢價(jià)  
10 mg   詢價(jià)  

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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 452 篇科研文獻(xiàn)

WB
IF
Proliferation Assay

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nature. 2022 Aug;608(7922):413-420.  [Abstract]

    Huh7 cells and ASGR1 KO cells are treated with 20?nM Bafilomycin A1 for 2?h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844.  [Abstract]

    Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30?nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844.  [Abstract]

    Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30?nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Nat Nanotechnol. 2022 Sep;17(9):993-1003.  [Abstract]

    Immunofluorescence (IF) images of phagocytosis and elimination of SC2-P (red) in the absence or presence of CIPS in THP-1 differentiated macrophages. The lysosomal inhibitor Bafilomycin A1 (BM; 100?nM; 6?h) is added to macrophages 6?h before the end of culture.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    The levels of mitochondrial proteins including TIM23, TOM20, and TOM22 decreased along with decreased O-GlcNAcylation levels, which can be inhibited by BafA1.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Mil Med Res. 2022 Feb 14;9(1):9.  [Abstract]

    GL261 and U251 cells are treated with 3-MA (5 mM), BafA1 (10 nM), AG-205 (10 μM), or RAPA (20 nM) for 1 h followed by IR or IR plus UTMD treatment for another 24 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Biomaterials. 2022 Sep;288:121743.

    In response to the Bafilomycin A1 (Baf; 50 nM) treatment, increases LC3 II expression is observed in PDLSCs, and significant differences between the Baf-treated PDLSCs and cells without the Baf treatment are observed at 6 h and 12 h during osteogenic induction.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Biomaterials. 2022 Sep;288:121743.

    Bafilomycin A1 (Baf; 50 nM) treatment significantly increases the percentage of both RFP+-GFP+-LC3 puncta and LC3 puncta in PDLSCs at 12 and 24 h during osteogenic induction, while there is no significant difference in the percentage of RFP+-GFP+-LC3 puncta or LC3 puncta between the Baf-treated I-PDLSCs and cells without the Baf treatment.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Feb 17;6(1):67.  [Abstract]

    Representative images of cells with GFP or GFP-LC3 puncta, and the average number of GFP-LC3 puncta per cell in stable RFP-GFP-LC3 U87 cells treated with Justicidin A (JA) (0.13?μM), Justicidin B (JB) (0.13?μM), or Justicidin C (JC) (4?μM) in the presence or absence of Compound C (2.5?μM), SCH772984 (10?μM), LY294002 (25?μM), or Bafilomycin A1 (1?nM) for 24?h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Metab. 2021 May 4;33(5):971-987.e6.  [Abstract]

    Western blot detection of YAP expression in HEK293T cells transfected with siControl or siATP6V0d2, followed by treatment with 100 nM Bafilomycin A1 (BafA1) for 6 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Stem Cell. 2021 Jun 3;28(6):1074-1089.e7.  [Abstract]

    Viability determined by cell counts of P, SA, and SAR iPSC-HSPCs treated with the V-ATPase inhibitor Bafilomycin A1 (Baf A1; 10 nM; 48 hours) compared with untreated cells.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Mol Immunol. 2022 Jan;19(1):67-78.  [Abstract]

    HeLa cells transfected with the ORF10-GFP plasmid and pEGFP-N1 are stimulated by poly(I:C) transfection and are then treated with MG132 (5?μM), Bafilomycin A1 (Baf A1; 5?nM), or Chloroquine (CQ; 40?μM) for 24?h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: J Extracell Vesicles. 2021 Oct;10(12):e12153.  [Abstract]

    Confocal microscopy analysis of the MVB marker CD63 in Coro1a‐Flag or Coro1a‐K233R‐Flag overexpressing HeLa cells treated with DMSO or 20 nM Bafilomycin A1 (Baf A1) for 12 h.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 3;9(10):1015.  [Abstract]

    Representative western blot images of LC3 (LC3I and LC3II) in primary PTC are isolated from WT and TRPC6-/- mice after treatment with H2O2 (0.5?mM 12?h) in the presence and absence of Bafilomycin A1 (BAF) (20?nM).

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195.  [Abstract]

    Western analysis of the effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without Bafilomycin A1 (Baf, 10 nM) in HEK293T cells.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195.  [Abstract]

    Western analysis of the effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without Bafilomycin A1 (Baf, 10 nM) in osteoclasts.

    Bafilomycin A1 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing SQSTM1 and LC3 levels in LN229 cells after treatment with 200?μM NSC 697855 (NTZ) and 200?nM Bafilomycin A1 (BAF) for 48?h.

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Bafilomycin A1 (BafA1) is a specific and reversible inhibitor of vacuolar H+-ATPase (V-ATPase) with IC50 values of 4-400 nmol/mg. Bafilomycin A1, a macrolide antibiotic, is also used as an autophagy inhibitor at the late stage. Bafilomycin A1 blocks autophagosome-lysosome fusion and inhibits acidification and protein degradation in lysosomes of cultured cells. Bafilomycin A1 induces apoptosis[1][2][3].

    IC50 & Target

    Macrolide

     

    體外研究
    (In Vitro)

    Bafilomycin A1 is treated to different types of membrane ATPases with the I50 of 400 nmol/mg, 4 nmol/mg and 50 nmol/mg for the vacuolar ATPases of a fungus (N. crassa), a plant (Z. mays), and an animal (bovine abrenal medulla). The I50 values refer as μmol of Bafilomycin A1 per mg of protein giving 50% inhibition of ATPase activity[1].
    Bafilomycin A1 ((-)-Bafilomycin A1) disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion[2].
    Bafilomycin A1 at a low concentration (1 nM) effectively and specifically inhibits and kills pediatric B-cell acute lymphoblastic leukemia cells. It targets both early and late stages of the autophagy pathway, mitochondria and induces caspase-independent apoptosis. Bafilomycin A1 induces the binding of Beclin 1 to Bcl-2, which further inhibits autophagy and promotes apoptotic cell death[5].
    The growth of the BEL-7402 hepatocellular carcinoma and HO-8910 ovarian cancer cell lines are retarded and the metastatic potential is inhibited by Bafilomycin A1. Transmission electron microscopy and assays of capsase-3 and -9 suggest that Bafilomycin A1 induces apoptosis[6].
    Bafilomycin A1 inhibits the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they are transformed, and PC12 and HeLa cells. The IC50 of Bafilomycin A1 for inhibition of cell growth ranges from 10 to 50 nM[7].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Chronic treatment with low-dose Bafilomycin A1 (0.1 mg/kg) slightly inhibits the tumor volume, but the final tumor volume does not differ significantly from the control. However, chronic treatment with high dose Bafilomycin A1 (1 mg/kg) inhibits the tumor growth significantly, compared with controls, after 21 days[8].
    Bafilomycin A1 (0.1 mg/kg or 1 mg/kg; i.p. daily for 3 days) extends the survival of B-cell acute lymphoblastic leukemia (B-ALL) xenograft mice with advanced disease[9].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    622.83

    Formula

    C35H58O9
    CAS 號(hào)
    性狀

    固體

    顏色

    White to light yellow

    中文名稱

    巴佛洛霉素A1；巴弗洛霉素A1
    結(jié)構(gòu)分類
    初始來源

    Streptomyces griseus strains
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 50 mg/mL (80.28 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.6056 mL 8.0279 mL 16.0557 mL
    5 mM 0.3211 mL 1.6056 mL 3.2111 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (4.01 mM); 懸濁液; 超聲助溶

      此方案可獲得 2.5 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      20% SBE-β-CD in Saline 的配制（4°C，儲(chǔ)存一周）：2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.34 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
    請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
    請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.43%

    參考文獻(xiàn)
    Cell Assay
    [2]

    Cells are harvested using 0.05% trypsin and suspended in culture medium containing 10% FCS, and 200 μL suspension is added to each well of a 96-well plate. Cells are cultured for 20 h for adhesion. Bafilomycin A1 is added to the wells at the final concentrations of 200, 400 and 800 nM, in triplicate. At 24, 48 and 72 h, 20 μl WST-1 is added to the cells. Following incubation at 37°C for 4 h, the plates are read to determine the optical density (OD) at 435 nm with 675 nm reference using a spectrophotometer[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [4]

    Mice: Tumor-bearing mice are divided randomly into three experimental groups: a low-dose Bafilomycin A1 (0.1 mg/kg per day)-treated group (n=5), a high-dose Bafilomycin A1 (1 mg/kg per day)-treated group (n=5),and a control group (n=5). Tumor size is measured and tumor volume doubling time is calculated[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6056 mL 8.0279 mL 16.0557 mL 40.1394 mL
    5 mM 0.3211 mL 1.6056 mL 3.2111 mL 8.0279 mL
    10 mM 0.1606 mL 0.8028 mL 1.6056 mL 4.0139 mL
    15 mM 0.1070 mL 0.5352 mL 1.0704 mL 2.6760 mL
    20 mM 0.0803 mL 0.4014 mL 0.8028 mL 2.0070 mL
    25 mM 0.0642 mL 0.3211 mL 0.6422 mL 1.6056 mL
    30 mM 0.0535 mL 0.2676 mL 0.5352 mL 1.3380 mL
    40 mM 0.0401 mL 0.2007 mL 0.4014 mL 1.0035 mL
    50 mM 0.0321 mL 0.1606 mL 0.3211 mL 0.8028 mL
    60 mM 0.0268 mL 0.1338 mL 0.2676 mL 0.6690 mL
    80 mM 0.0201 mL 0.1003 mL 0.2007 mL 0.5017 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Bafilomycin A1
    目錄號(hào)：
    HY-100558
    需求量：