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  4. Myelin Basic Protein (MBP) (68-82), guinea pig

Myelin Basic Protein (MBP) (68-82), guinea pig 

目錄號(hào): HY-P1048 純度: 98.75%
COA 產(chǎn)品使用指南

Myelin Basic Protein (MBP) (68-82), guinea pig 是髓鞘堿性蛋白 (MBP) 的一個(gè)片段。

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Custom Peptide Synthesis

Myelin Basic Protein (MBP) (68-82), guinea pig Chemical Structure

Myelin Basic Protein (MBP) (68-82), guinea pig Chemical Structure

CAS No. : 98474-59-0

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1 mg ¥700
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5 mg ¥1500
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10 mg ¥2400
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25 mg ¥4213
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Customer Review

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Myelin Basic Protein (MBP) (68-82), guinea pig is a fragment of myelin basic protein (MBP).

體外研究
(In Vitro)

多發(fā)性硬化癥是最常見的影響中樞神經(jīng)系統(tǒng)的自身免疫性疾病。在這項(xiàng)研究中,分析了全血樣本的激活能力以及 CD4+ 和 CD8+ T 淋巴細(xì)胞被人總髓鞘堿性蛋白 (MBP)、人 MBP 激活的能力104-118 片段和豚鼠 MBP (68-82) 片段。在全血中觀察到活化的 T 淋巴細(xì)胞數(shù)量顯著增加。對(duì)于所有三種測(cè)試的 MBP,活化的 CD4+ 和 CD8+ T 淋巴細(xì)胞的增加具有統(tǒng)計(jì)學(xué)意義 (p<0.01)。然而,活化 T 細(xì)胞的這種增加在與人類總 MBP 孵育后最為顯著,其次是人類 104-118 片段;在與豚鼠 MBP (68-82) 片段孵育后觀察到最小的增加 (人總 MBP>huMBP-104-118>豚鼠 MBP (68-82) )[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

研究在 MBP (68-82) 免疫當(dāng)天用蜂毒針灸 (BVA) 進(jìn)行預(yù)處理是否會(huì)影響實(shí)驗(yàn)性自身免疫性腦脊髓炎 (EAE) 的誘發(fā)和進(jìn)展以及體重減輕。 免疫后 5-9 天,髓鞘堿性蛋白 (MBP) 組大鼠在自由移動(dòng)環(huán)境中開始出現(xiàn)尾部緊張度部分喪失 (臨床癥狀,0.5)。 免疫后 10-16 天,MBP 組大多數(shù)大鼠出現(xiàn)更嚴(yán)重的神經(jīng)功能缺損癥狀,包括后肢截癱、截癱、四肢輕癱和四肢癱瘓。相比之下,與 MBP 組大鼠相比,MBP?+?BVA 組大鼠在免疫后 11-15 天以劑量依賴性方式表現(xiàn)出相對(duì)輕微的神經(jīng)功能缺損。與 MBP 組相比,癥狀出現(xiàn)時(shí)間略有延遲(BVA 0.8 mg/kg,6.4±0.6 天),最大臨床評(píng)分明顯降低(BVA 0.25 mg/kg,3.7±0.2;BVA 0.8 mg/kg,2.8±0.3)。此時(shí),MBP 組大鼠的平均體重與正常組大鼠相比有所下降,但 MBP?+?BVA 組大鼠的平均體重與 MBP 組大鼠相比有所增加[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1736.79

Formula

C71H113N23O28

CAS 號(hào)
性狀

固體

顏色

White to off-white

Sequence

Tyr-Gly-Ser-Leu-Pro-Gln-Lys-Ser-Gln-Arg-Ser-Gln-Asp-Glu-Asn

Sequence Shortening

YGSLPQKSQRSQDEN

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn): 

H2O 中的溶解度 : 50 mg/mL (28.79 mM; 超聲助溶)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 0.5758 mL 2.8789 mL 5.7577 mL
5 mM 0.1152 mL 0.5758 mL 1.1515 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?個(gè)月內(nèi)使用,-20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?個(gè)月內(nèi)使用。

* 備注:如您選擇水作為儲(chǔ)備液,請(qǐng)稀釋至工作液后,再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn):

以下溶解方案,請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配,在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比; 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過(guò)加熱和/或超聲的方式助溶。

  • 方案 一

    請(qǐng)依序添加每種溶劑: PBS

    Solubility: 100 mg/mL (57.58 mM); 澄清溶液; 超聲助溶

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息:

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

由于實(shí)驗(yàn)過(guò)程有損耗,建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳,請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度,如有需要,請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Kinase Assay
[1]

For each individual, the whole blood sample is typically divided into six 1 mL aliquots/tubes. Concanavalin A is added to tube 1 (positive control). Tube 2 is left untreated. Tube 3 is treated with human albumin as a negative control. Human total MBP, human MBP 104-118 fragment and guinea pig MBP (68-82) are added to tubes 4, 5 and 6, respectively. All proteins are added to a final concentration of 2 μg/mL. All experiments (incubations and flow cytometric analysis) are performed in duplicate for each subject[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Rats[2]
Ten-week-old female Lewis rats are divided into the following two experimental groups: BVA-pretreated (every 3 days from 20 min before immunization) and BVA-posttreated (daily from days 10-15 after immunization) groups. Each experimental group is subdivided into the following five groups: normal [saline, subcutaneous (s.c.)+saline, s.c.], MBP [250 μg of myelin basic protein MBP (68-82), s.c.+saline, s.c., ST36 acupoint], MBP+BVA 0.25 [250 μg of MBP (68-82), s.c.+0.25 mg/kg body weight of BV, s.c., ST36 acupoint], MBP+BVA 0.8 [250 μg of MBP (68-82), s.c.+0.8 mg/kg body weight of BV, s.c., ST36 acupoint], and BVA alone [saline, s.c.+0.8 mg/kg body weight of BV, s.c., ST36 acupoint] groups. EAE is induced with an emulsion containing 250 μg of MBP (68-82) and 100 μg of Mycobacterium M. tuberculosis per mL of incomplete Freund’s adjuvant. A total of 0.2 mL of this emulsion is injected s.c. into the two hind footpads of rats except for those in the normal group and BVA alone group. Additionally, rats receive intraperitoneal (i.p.) injections of 200 ng of pertussis toxin on days 0 and 2. Rats in the normal group are treated with saline alone instead of MBP (68-82) peptide, pertussis toxin, or BVA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻(xiàn)

Myelin Basic Protein (MBP) (68-82), guinea pig 相關(guān)分類

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效
儲(chǔ)備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?個(gè)月內(nèi)使用,-20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.5758 mL 2.8789 mL 5.7577 mL 14.3944 mL
5 mM 0.1152 mL 0.5758 mL 1.1515 mL 2.8789 mL
10 mM 0.0576 mL 0.2879 mL 0.5758 mL 1.4394 mL
15 mM 0.0384 mL 0.1919 mL 0.3838 mL 0.9596 mL
20 mM 0.0288 mL 0.1439 mL 0.2879 mL 0.7197 mL
25 mM 0.0230 mL 0.1152 mL 0.2303 mL 0.5758 mL

* 備注:如您選擇水作為儲(chǔ)備液,請(qǐng)稀釋至工作液后,再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
Myelin Basic Protein (MBP) (68-82), guinea pig
目錄號(hào):
HY-P1048
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