The effect of tangeretin on RAW264.7 cells was determined using a MTT assay as previously reported.(13) Briefly, RAW264.7 cells (1 × 104 cells/well) were seeded in a 96-well plate for 24 h and treated with different concentrations of tangeretin (6.3–50.0 μM) and dimethyl sulfoxide (DMSO) (vehicle control, 0.01 and 0.1%) for 10 or 48 h. The absorbance was measured at 570 nm using an enzyme immunoassay (EIA) reader (Thermo Scientific, Waltham, MA), and cell viability (%) was calculated as follows: [(absorbance of the test group – absorbance of the blank control)/(absorbance of the control group – absorbance of the blank control)] × 100.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal administration [2] The mice were maintained in an air-conditioned, pathogen-free room (temperature of 24 ± 2 °C, with a 12 h light/dark cycle from 6:00 am to 6:00 pm) with free access to food and water. Mice were randomly divided into five groups (n = 10) as follows: normal (control), RSV-challenged, and three treatment groups administered 25, 50, or 100 mg/kg/day tangeretin dissolved in saline. The control and RSV-challenged groups received equal volumes of saline. During the experiment, mice in the treatment groups were intragastrically administrated tangeretin for 3 days consecutively before RSV stimulation. Mice were lightly anesthetized with diethyl ether and intranasally challenged with RSV Long strain [6.7 × 106 plaque-forming units (PFU)] on day 4 after tangeretin treatment, while the control group was sham-infected with an equal volume of HEp-2 cell lysate, which was centrifuged under the same conditions as the viral suspensions. The mice were weighed during the experiment and sacrificed on day 5 post-infection after anesthetizing them with chloral hydrate (Figure 1B). The lung tissues were removed and weighed, and the lung index was calculated using the following formula: lung index = lung weight/body weight.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Zhang X, et al. Tangeretin enhances radiosensitivity and inhibits the radiation-induced epithelial-mesenchymal transition of gastric cancer cells. Oncol Rep. 2015 Jul;34(1):302-10.  [Content Brief]

  [2]. Xu JJ, et al. Tangeretin from Citrus reticulate Inhibits Respiratory Syncytial Virus Replication and Associated Inflammation in Vivo. J Agric Food Chem. 2015 Nov 4;63(43):9520-7.  [Content Brief]

  [3]. Hagenlocher Y, et al. Citrus peel polymethoxyflavones nobiletin and tangeretin suppress LPS- and IgE-mediated activation of human intestinal mast cells. Eur J Nutr. 2017 Jun;56(4):1609-1620.  [Content Brief]