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  2. Metabolic Enzyme/Protease
  3. E1/E2/E3 Enzyme
  4. Ginkgolic Acid

Ginkgolic Acid  (Synonyms: 銀杏酸; Ginkgolic acid (15:1); Ginkgolic acid I; Romanicardic acid)

目錄號: HY-N0077 純度: 99.92%
COA 產(chǎn)品使用指南 技術(shù)支持

Ginkgolic Acid是一種天然化合物， 在體外實驗中抑制 SUMOylation 的 IC50 為3.0 μM。

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Ginkgolic Acid Chemical Structure

Ginkgolic Acid Chemical Structure

CAS No. : 22910-60-7

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥1364
In-stock
1 mg ￥624
In-stock
5 mg ￥1240
In-stock
10 mg ￥2040
In-stock
50 mg   詢價  
100 mg   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Ginkgolic Acid:

    Ginkgolic Acid purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Apr 15;345:1-9.  [Abstract]

    Ginkgolic acid (GA) reduces cardiac fibrosis and inflammation in ventricular tissue of MI mice. SUMO-1 measured with Western blot. GA: 50 mg/kg per day.

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Ginkgolic Acid is a natural compound that inhibits SUMOylation with an IC50 of 3.0 μM in in vitro assay.

    IC50 & Target

    IC50: 3.0 μM (SUMOylation)[1]
    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    31.3 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human A549 cells after 2 days by SRB assay
    Growth inhibition of human A549 cells after 2 days by SRB assay
    		[PMID: 9677265]
    CCD-18Co IC50
    97.14 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human CCD-18Co cells after 2 days by SRB assay
    Growth inhibition of human CCD-18Co cells after 2 days by SRB assay
    		[PMID: 9677265]
    HCT-15 IC50
    12.7 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human HCT15 cells after 2 days by SRB assay
    Growth inhibition of human HCT15 cells after 2 days by SRB assay
    		[PMID: 9677265]
    HT1197 IC50
    46.26 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human HT1197 cells after 2 days by SRB assay
    Growth inhibition of human HT1197 cells after 2 days by SRB assay
    		[PMID: 9677265]
    MCF7 IC50
    6.44 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human MCF7 cells after 2 days by SRB assay
    Growth inhibition of human MCF7 cells after 2 days by SRB assay
    		[PMID: 9677265]
    SK-OV-3 IC50
    > 50 μg/mL
    Compound: 5, phenolic acid C15:1
    Growth inhibition of human SKOV3 cells after 2 days by SRB assay
    Growth inhibition of human SKOV3 cells after 2 days by SRB assay
    		[PMID: 9677265]
    體外研究
    (In Vitro)

    Ginkgolic acid inhibits the in vitro SUMOylation of RanGAP1-C2 with the IC50 values of 3.0 μM. The level of SUMOylated p53 is markedly reduced by the ginkgolic acid treatment. Importantly, ginkgolic acid does not affect protein ubiquitination in cells. Ginkgolic acid inhibits the binding between E1 and GA-BODIPY in a dose-dependent manner[1]. Ginkgolic acid (31.2 μg/mL) inhibits HIV protease activity by 60%, compared with the negative control, and the effect is concentration-dependent. Ginkgolic acid treatment (50 and 100 μg/mL) effectively inhibits HIV infection in human PBMC cells. Ginkgolic acid at the concentrations up to 150 μg/mL does not cause any significant cytotoxicity in Jurkat cells[2]. GA only inhibits the growth of tumorogenic cell lines in a both dose- and time-dependent manner. Tumor cells are treated with GA for 72 h, 70.53±4.54% Hep-2 and 63.5±7.2% Tca8113 cells are retarded at GO/G1 phase, and the percentage of apoptosis is 40.4±1.58 and 38.4±1.7%, respectively. GA-treated activated caspase-3 downregulates the expression of anti-apoptotic Bcl-2 protein and upregulates the expression of pro-apoptotic Bax protein, eventually leading to a decrease in the Bcl-2/Bax ratio in tumor cellsin human PBMC cells. Ginkgolic acid at the concentrations up to 150 μg/mL does not cause any significant cytotoxicity in Jurkat cells[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    346.50

    Formula

    C22H34O3
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    銀杏酸；白果酸
    結(jié)構(gòu)分類
    初始來源
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (288.60 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.8860 mL 14.4300 mL 28.8600 mL
    5 mM 0.5772 mL 2.8860 mL 5.7720 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.75 mg/mL (7.94 mM); 澄清溶液

      此方案可獲得 ≥ 2.75 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 27.5 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% Corn Oil

      Solubility: ≥ 2.75 mg/mL (7.94 mM); 澄清溶液

      此方案可獲得 ≥ 2.75 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。

      以 1 mL 工作液為例，取 100 μL 27.5 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.92%

    參考文獻
    Cell Assay
    [2]

    Jurkat cells (106?cells/mL) are cultured in the RPMI medium with or without different concentrations of ginkgolic acid for 48 hours to test the cytotoxicity of ginkgolic acid. The cytotoxicity of ginkgolic acid is determined using a tetrazolium compound (MTS) and an electron coupling reagent (PMS). MTS is chemically reduced by cells into formazan, which is soluble in the tissue culture medium. The measurement of the absorbance of the formazan can be carried out using 96 well microplates at 492 nm. Since the production of formazan is proportional to the number of living cells, the intensity of the produced color is a good indication of the viability of the cells.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8860 mL 14.4300 mL 28.8600 mL 72.1501 mL
    5 mM 0.5772 mL 2.8860 mL 5.7720 mL 14.4300 mL
    10 mM 0.2886 mL 1.4430 mL 2.8860 mL 7.2150 mL
    15 mM 0.1924 mL 0.9620 mL 1.9240 mL 4.8100 mL
    20 mM 0.1443 mL 0.7215 mL 1.4430 mL 3.6075 mL
    25 mM 0.1154 mL 0.5772 mL 1.1544 mL 2.8860 mL
    30 mM 0.0962 mL 0.4810 mL 0.9620 mL 2.4050 mL
    40 mM 0.0722 mL 0.3608 mL 0.7215 mL 1.8038 mL
    50 mM 0.0577 mL 0.2886 mL 0.5772 mL 1.4430 mL
    60 mM 0.0481 mL 0.2405 mL 0.4810 mL 1.2025 mL
    80 mM 0.0361 mL 0.1804 mL 0.3608 mL 0.9019 mL
    100 mM 0.0289 mL 0.1443 mL 0.2886 mL 0.7215 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Ginkgolic Acid
    目錄號：
    HY-N0077
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