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  2. Anti-infection Autophagy
  3. Parasite Influenza Virus Autophagy
  4. Nitazoxanide

Nitazoxanide  (Synonyms: 硝唑尼特; NTZ; NSC 697855)

目錄號: HY-B0217 純度: 99.95%
COA 產(chǎn)品使用指南

Nitazoxanide (NTZ) 是一種廣譜驅(qū)蟲劑 (anthelmintic)，對感染動物和人類的各種蠕蟲、原生動物和腸道細菌都具有作用活性。 Nitazoxanide 在無菌培養(yǎng)中抑制 Giardia lamblia 滋養(yǎng)體增殖，IC50 為 2.4 μM。Nitazoxanide 可用于寄生蟲性 (parasitic) 胃腸炎的研究。Nitazoxanide還具有抗病毒特性。 Nitazoxanide 在小鼠模型中顯示出抗日本腦炎病毒 (JEV) 活性。

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Nitazoxanide Chemical Structure

Nitazoxanide Chemical Structure

CAS No. : 55981-09-4

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥500
In-stock
1 mg ￥140
In-stock
5 mg ￥281
In-stock
10 mg ￥450
In-stock
50 mg ￥1469
In-stock
100 mg ￥1989
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Nitazoxanide:

    Nitazoxanide purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing the cell cycle and autophagy-related proteins after 48?h of treatment with 200?μM Nitazoxanide (NTZ) and/or 500?nM Torin 1 or 30?mM Chloroquine (CQ).

    Nitazoxanide purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032.  [Abstract]

    Western blot showing SQSTM1 and LC3 levels in LN229 cells after treatment with 200?μM Nitazoxanide (NTZ) and 200?nM Bafilomycin A1 (BAF) for 48?h.

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model[2].

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    > 50 μM
    Compound: NTZ
    Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    BHK-21 CC50
    25 μM
    Compound: 105
    Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
    Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
    		[PMID: 28689975]
    Caco-2 IC50
    221 μM
    Compound: Nitazoxanide
    Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry
    Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry
    		[PMID: 28601526]
    Caco-2 IC50
    26.8 μM
    Compound: NTZ
    Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    HEK-293T CC50
    > 40 μM
    Compound: Nitazoxanide
    Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
    Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
    		[PMID: 29469575]
    HeLa IC50
    35 μM
    Compound: NTZ
    Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    HeLa IC50
    35 μM
    Compound: NTZ
    Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    HepG2 CC50
    > 40 μM
    Compound: Nitazoxanide
    Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
    Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay
    		[PMID: 29469575]
    HepG2 2.2.15 CC50
    > 100 μM
    Compound: 1, NTZ
    Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay
    Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay
    		[PMID: 21553812]
    HL-60 IC50
    20.1 μM
    Compound: NTZ
    Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    HT-29 IC50
    > 50 μM
    Compound: NTZ
    Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    Huh-7 CC50
    49 μM
    Compound: 1, NTZ, PH-5776
    Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay
    Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay
    		[PMID: 22059983]
    PC-3 IC50
    44.7 μM
    Compound: NTZ
    Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
    		[PMID: 34055214]
    Peritoneal macrophage IC50
    2 mM
    Compound: 36, NTZ
    Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis
    Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis
    		[PMID: 32992245]
    RAW264.7 IC50
    1.54 mM
    Compound: 36, NTZ
    Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis
    Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis
    		[PMID: 32992245]
    U2OS CC50
    25 μM
    Compound: 105
    Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
    Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay
    		[PMID: 28689975]
    Vero IC50
    10.74 μM
    Compound: NTZ
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay
    		[PMID: 23787289]
    Vero CC50
    833 μM
    Compound: Nitazoxanide
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
    		[PMID: 25801157]
    Vero CC50
    833 μM
    Compound: NIT, Alinia
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    		[PMID: 21397502]
    Vero CC50
    833 μM
    Compound: Nitazoxanide
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
    		[PMID: 28645659]
    Vero CC50
    833 μM
    Compound: NTZ
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
    		[PMID: 24529307]
    Vero C1008 CC50
    > 35.5 μM
    Compound: 48
    Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay
    Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay
    		[PMID: 32845145]
    體外研究
    (In Vitro)

    Giardia lamblia, a flagellated protozoan, is the most common causative agent of persistent diarrhea worldwide[1].
    ? Nitazoxanide exhibits effect on G. lamblia trophozoite proliferation in axenic culture with an IC50 of 2.4 μM[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (103 to 106 parasites per well)
    Concentration: 30 μM
    Incubation Time: 24 hours
    Result: 70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 105 parasites per well.
    The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 105 trophozoites.
    體內(nèi)研究
    (In Vivo)

    Nitazoxanide exhibits a wide spectrum of in vivo activity against a broad spectrum of intestinal parasites, such as Giardia lamblia, Entamoeba histolytica, Trichomonas vaginalis, the apicomplexan Cryptosporidium parvum, and enteric bacteria infecting animals and humans[1].
    ? Nitazoxanide (50, 75 or 100 mg/kg/day; administered daily by intragastric for up to 25 days) reduces the mortality of Japanese encephalitis virus (JEV) strain-infected mice and protected mice from a lethal dose challenge of JEV[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV[2]
    Dosage: 50, 75 or 100 mg/kg/day
    Administration: Administered intragastrically by gavage
    Result: 50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.
    分子量

    307.28

    Formula

    C12H9N3O5S
    CAS 號
    性狀

    固體

    顏色

    Light yellow to yellow

    中文名稱

    硝唑尼特
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : ≥ 100 mg/mL (325.44 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 3.2544 mL 16.2718 mL 32.5436 mL
    5 mM 0.6509 mL 3.2544 mL 6.5087 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.25 mg/mL (10.58 mM); 澄清溶液

      此方案可獲得 ≥ 3.25 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 32.5 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.95%

    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2544 mL 16.2718 mL 32.5436 mL 81.3590 mL
    5 mM 0.6509 mL 3.2544 mL 6.5087 mL 16.2718 mL
    10 mM 0.3254 mL 1.6272 mL 3.2544 mL 8.1359 mL
    15 mM 0.2170 mL 1.0848 mL 2.1696 mL 5.4239 mL
    20 mM 0.1627 mL 0.8136 mL 1.6272 mL 4.0680 mL
    25 mM 0.1302 mL 0.6509 mL 1.3017 mL 3.2544 mL
    30 mM 0.1085 mL 0.5424 mL 1.0848 mL 2.7120 mL
    40 mM 0.0814 mL 0.4068 mL 0.8136 mL 2.0340 mL
    50 mM 0.0651 mL 0.3254 mL 0.6509 mL 1.6272 mL
    60 mM 0.0542 mL 0.2712 mL 0.5424 mL 1.3560 mL
    80 mM 0.0407 mL 0.2034 mL 0.4068 mL 1.0170 mL
    100 mM 0.0325 mL 0.1627 mL 0.3254 mL 0.8136 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Nitazoxanide
    目錄號：
    HY-B0217
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